PERFORMANCE OF LIQUID HELIUM ELECTRON CRYOMICROSCOPE (JEOL3000SFF)

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。JEOL 3000 SFF低温电子显微镜是一种液氦显微镜，工作电压300 kV，样品温度4.2K。该仪器目前专用于高分辨率单粒子图像数据收集。我们有两名训练有素的科学家经常使用这种仪器。该仪器在一年中的大部分时间都处于高性能模式。停机时间主要是由于物镜孔径脏、相机堵塞和自动样品盒装载装置故障。生物学项目包括二十面体病毒颗粒和伴侣蛋白颗粒，其目标是4？分辨率数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The JEOL3000SFF electron cryomicroscope is a liquid helium microscope opearted at 300 kV and specimen temperature of 4.2K. This instrument is presently dedicated to high resolution single particle image data collection. We have 2 well-trained scientists using this instrument routinely. The instrument has been in high performance mode throughout most of the year. The downtimes have attributable mostly to the dirty objective aperture, camera jam and malfunction of the automated specimen cartiledge loading device. The biolgoical projects include icosahedral virus particles and chaperonin particles which are aimed at 4 ¿ resolution data.