IN VIVO DTI OF RAT BRAIN ON A 3T MR SYSTEM W/INSERT GRADIENTS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。介绍：物质依赖的动物模型可以促进识别成瘾的易感性，发展和维持的生物学机制。与人类酒精成瘾特别相关的是大脑白色物质，死后研究表明，它会受到髓鞘和细胞骨架降解的影响，这在戒酒后可能是可逆的。酒精依赖的体内人体扩散张量成像（DTI）研究提供了常规MRI体积测量无法检测到的白色物质微观结构破坏的证据。在这里，我们报告的发展，在体内回波平面（EP）扩散张量成像（DTI）协议的3T人类MRI系统配备了高强度插入梯度线圈的研究大鼠大脑。方法和讨论：用GE 3T磁共振扫描仪对健康成年Wistar大鼠进行扫描，扫描线圈为高强度插入梯度线圈（500mT/m，1800T/m/s）和定制正交鸟笼线圈（？）= 44 mm）。成像采用EP DTI方案，各向同性分辨率为0.5mm。在6个非共线方向上应用扩散加权，b值= 1009 s/mm2。分数各向异性（FA）图像具有足够的信噪比来检测大鼠脑白色物质结构。可以在图像中识别纤维的主要取向，其中FA值乘以最大特征值的特征向量的主分量（x，y和z）。 资金来源R37 AA005965，<$CNS缺陷：年龄和酒精的相互作用<$，Adolf Pfefferbaum，1997年5月1日-2006年6月30日<$R01 AA012388，<$酒精中毒的体内弥散和光谱脑成像<$，Adolf Pfefferbaum，2000年8月1日-2010年6月30日<$U01 AA013521，<$INIA：成像核心Adolf Pfefferbaum，2001年9月27日至2006年8月31日 相关出版物：E.V. Sullivan，E.阿达尔斯泰因松河Sood，D. Mayer，R.L. Bell，W. McBride，T. K. Li，长穗条锈菌A. Pfefferbaum <$Part I--Alcohol-Prefering（P）Rat的纵向脑MRI研究：成年脑生长<$Alcohol Clin. Exp.结果：审查中。是的Pfefferbaum，E.阿达尔斯泰因松河Sood，D. Mayer，R.L. Bell，W. McBride，T. K.李，E.V.沙利文第二部分-纵向脑磁共振成像研究的酒精偏好（P）大鼠：自愿慢性酒精消费的影响<$，酒精临床。结果：审查中。D. Mayer，E. Adalsteinsson，B.K. Rutt，N. Zahr，E.V. Sullivan，A.普费弗鲍姆使用3T临床MRI系统和高强度插入梯度线圈采集的大鼠脑的体内扩散张量成像，Proc ISMRM，第14届年会，西雅图，2006年。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Animal models of substance dependence can facilitate the identification of biological mechanisms underlying the predisposition, development, and maintenance of addiction. Of particular relevance to human alcohol addiction is brain white matter, which postmortem study has shown is subject to myelin and cytoskeletal degradation that may be reversible with abstinence. In vivo human diffusion tensor imaging (DTI) studies of alcohol dependence provide evidence for microstructural disruption of white matter not detectible with bulk volume measures of conventional MRI. Here, we report on the development of an in vivo echo-planar (EP) diffusion tensor imaging (DTI) protocol on a 3T human MRI system equipped with a high-strength insert gradient coil for study of rat brain. Methods and Discussion: Healthy adult Wistar rats were scanned in a clinical GE 3T MR scanner equipped with a high-strength insert gradient coil (500mT/m, 1800T/m/s) and a custom quadrature birdcage coil (? = 44 mm). Imaging was accomplished with an EP DTI protocol for 0.5mm isotropic resolution. Diffusion weighting was applied for b-value=1009 s/mm2 in 6 noncollinear directions. Fractional anisotropy (FA) images had adequate signal-to-noise ratio to detect rat brain white matter structures. The primary orientation of the fibers could be identified in images where the FA value is multiplied by the principal component (x, y, and z) of the eigenvector of the largest eigenvalue. Funding Sources ¿ R37 AA005965, ¿CNS DEFICITS: INTERACTION OF AGE AND ALCOHOLISM¿, Adolf Pfefferbaum, 5/1/1997-6/30/2006 ¿ R01 AA012388, ¿In Vivo Diffusion and Spectroscopic Brain Imaging in Alcoholism¿, Adolf Pefferbaum, 8/1/2000-6/30/2010 ¿ U01 AA013521, ¿INIA: Imaging Core¿, Adolf Pfefferbaum, 9/27/2001-8/31/2006 Relevant publications ¿ E.V. Sullivan, E. Adalsteinsson, R. Sood, D. Mayer, R.L. Bell, W. McBride, T.-K. Li, A. Pfefferbaum ¿Part I--Longitudinal Brain MRI Study of the Alcohol-Preferring (P) Rat: Adult Brain Growth¿, Alcohol Clin. Exp. Res., in review. ¿ A. Pfefferbaum, E. Adalsteinsson, R. Sood, D. Mayer, R.L. Bell, W. McBride, T.-K. Li, E.V. Sullivan ¿Part II--Longitudinal Brain MRI Study of the Alcohol-Preferring (P) Rat: Effects of Voluntary Chronic Alcohol Consumption¿, Alcohol Clin. Exp. Res., in review. ¿ D. Mayer, E. Adalsteinsson, B.K. Rutt, N. Zahr, E.V. Sullivan, A. Pfefferbaum ¿In Vivo Diffusion Tensor Imaging of Rat Brain Acquired with a 3T Clinical MRI System and a High Strength Insert Gradient Coil¿, Proc ISMRM, 14th Annual Meeting, Seattle, 2006.