REGULATION OF IMMUNE CELL GLYCAN-RELATED GENE EXPRESSION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。参与小鼠糖蛋白生物合成、分解代谢和识别（凝集素）的各种酶和蛋白质的转录水平正在通过qRT-PCR进行定量。哈恩实验室的重点是免疫调节碳水化合物，特别是它们诱导th2型T细胞反应、激活巨噬细胞和树突状细胞以及驱动免疫抑制的能力。通过qRT-PCR对小鼠免疫细胞群进行糖相关基因表达分析，并将结果与不同方法诱导的细胞群进行比较，以确定存在于不同细胞群中的糖基转移酶和凝集素的库，以及它们的表达水平在免疫调节刺激后如何改变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcript levels for various enzymes and proteins involved in murine glycoprotein biosynthesis, catabolism, and recognition (lectins) are being quantitated by qRT-PCR. The focus of the Harn laboratory is on immunomodulatory carbohydrates, specifically in their ability to induce Th2-type T cell responses, activate macrophages, and dendritic cells and to also drive immune suppression. Mouse immune cell populations are being profiled for glycan-related gene expression by qRT-PCR and the results will be compared between cell populations induced by various approaches to determine the repertoire of glycosyltransferases and lectins that are present in different cell populations and how their expression levels are altered following immunomodulatory stimuli.