CORE--PROTEOMICS FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。Siteman癌症中心（SCC）蛋白质组学核心使用当前和新开发的方法和试剂为SCC成员提供多重蛋白质组学分析。这些方法包括使用多种荧光染色的二维差分凝胶电泳，用于定量和检测功能基团，使用串联质谱法（LC-MS/MS和MALDI-TOF/-TOF）进行蛋白质鉴定，以及使用差分稳定同位素标记的多维液相色谱方法。SCC蛋白质组学核心正在与SCC生物信息学核心（Jeffrey Milbrandt，核心主管）和多路基因分析核心（Mark Watson，核心主管）合作，建立一个实验室信息管理系统（LIMS）和一个关系数据库，使SCC研究人员能够通过一个单一的医学信息学门户访问和查询临床、蛋白质组学和mRNA阵列数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.