Host genes controlling Flavivirus infection: new insights and application for developing highly effective Kunjin replicon-based Ebola vaccine
Host genes controlling Flavivirus infection: new insights and application for developing highly effective Kunjin replicon-based Ebola vaccine
批准号:
nhmrc : 1102421
负责人:
Prof Alexander Khromykh
金额:
$50.62万
依托单位国家:
澳大利亚
项目类别:
Project Grants
财政年份:
2016
资助国家:
澳大利亚
项目状态:
已结题
起止时间:
2016-01-01 至 2018-12-31
中文摘要
这些应用程序旨在识别控制西尼罗河病毒和其他医学上重要的黄病毒(如登革热和日本脑炎)感染的新宿主基因。为此,我们将使用新的体内RNAi筛选方法,利用病毒文库编码针对整个小鼠基因组的人工microRNAs (amirs)。然后,我们将应用amiR技术生产高效的基于Kujniin复制的埃博拉候选疫苗,该疫苗在灵长类动物试验中显示出有希望的结果。
英文摘要
The applications is aimed at identifying new host genes controlling infection with West Nile virus and other medically important flaviviruses such as dengue and Japanese encephalitis. For this, we will use novel in vivo RNAi screening approach with virus libraries encoding artificial microRNAs (amirs) targeting whole mouse genome. We will then apply amiR technology to produce highly effective Kujniin replicon-based Ebola vaccine candidate that has shown promising results in trails in primates.
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