CORE--RESEARCH CYTOGENETICS

研究细胞遗传学和激光捕获显微切割(RCLC)设施提供 核型、分子细胞遗传学分析和激光捕获显微切割(LCM)。这个 RCLC基金成立于1995年7月，由联合国气候变化框架公约的11个分部的所有三个分部的成员使用 研究项目。在过去五年中，该机制资助了29名调查人员。在……里面 除了提供全方位的细胞遗传学服务外，RCLC设施还增加了用于结构重排分析的多重荧光原位杂交(M-FISH)和用于分析肿瘤标本和细胞系DNA拷贝数变化的阵列比较基因组杂交(ARRAY-CGH)。两个完整的LCM工作站现已上市，其中包括一台新的AutoPix LCM仪器(Arcturus)，它通过一个完全自动化的系统提供快速和方便的纯细胞群访问。LCM部分由训练有素的病理学家管理。设施主任是人类遗传学的项目负责人，细胞遗传学部分由一位经验丰富和熟练的细胞遗传学家管理。2003年，与1999年相比，研究细胞遗传学部门进行的测试次数增加了近四倍，当时这是一个发展中的核心基金。1999年，我们对20个样本进行了常规FISH定位、核型分析和CGH分析，而在2003年，我们分析了73个样本，增加了265%。自四年前康乐及文化事务署的设施首次提供流动电话服务以来，流动电话的使用量已由2000年的150小时增至2003年的570小时，增幅达280%。该机制的业务预算由用户费用提供支助的百分比从1999年的11%增加到2003年的20%。虽然对FISH定位本地基因座的需求实际上已经结束，但对FISH分析结构重排或病毒/转基因整合位点、间期FISH以识别未分裂细胞中的基因组变化以及染色体绘制和M-FISH以便于解释复杂或细微的染色体重排的要求增加了160%。此外，对小鼠模型研究中使用的胚胎干细胞克隆进行核型分析的请求是一种新的频繁需求，平均每年有24项请求。

The Research Cytogenetics and Laser Capture Microdissection (RCLC) Facility provides karyolyping, molecular cytogenetic analyses, and Laser Capture Microdissection (LCM). The RCLC Facility, established in July 1995, is used by Members of all three FCCC Divisions from 11 research Programs. The Facility supported 29 funded investigators over the last five years. In addition to offering a full spectrum of cytogenetic services, the RCLC Facility has added multiplex-fluorescence In situ hybridization FISH (M-FISH) for analysis of structural rearrangements and array-comparative genomic hybridization (array-CGH) for analysis of DNA copy number changes in tumor specimens and cell lines. Two complete LCM workstations are now available, including a new AutoPix LCM apparatus (Arcturus) that provides rapid and convenient access to pure cell populations through a totally automated system. The LCM component is managed by a trained pathologist. The Facility Director is the Program Leader of Human Genetics and the cytogenetics component is managed by an experienced and skilled cytogeneticist. In 2003, the number of tests performed by the Research Cytogenetics component increased nearly four-fold compared to that carried out in 1999, when this was a Developing Core Facility. In 1999, we performed conventional FISH mapping and karyotypic and CGH analyses on 20 samples, whereas in 2003, 73 samples were analyzed, an increase of 265%. From the time when LCM services were first introduced in the RCLC Facility four years ago, LCM usage has increased from 150 hours in 2000 to 570 hours in 2003, an increase of 280%. The percentage of the Facility's operating budget supported by user's fees increased from 11% in 1999 to 20% in 2003. Although demand for FISH mapping of native gene loci has virtually ended, requests increased by 160% for FISH analysis of structural rearrangements or viral/transgene integration sites, interphase FISH to identify genomic alterations in non-dividing cells, and chromosome painting and M-FISH to facilitate interpretation of complex or subtle chromosome rearrangements. In addition, requests for karyotyping of embryonic stem cell clones used in mouse model studies is a new frequent demand averaging 24 requests per year.