GLYCOPROTEIN GLYCOMICS: EXPRESSION IN MOUSE ES CELLS & PROGENY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Core 2A正在开发分析细胞糖蛋白糖组的工具，包括胚胎小鼠干细胞和 差异化的产品。采用系列凝集素亲和层析法分离胰蛋白酶糖肽， 正在开发通过表达的聚糖类型对表达进行分类。然后进一步分离糖肽 通过液相色谱法完成，然后通过质谱法对各种物种进行分类和鉴定。在 在各个阶段，N-聚糖酶处理将产生N-连接聚糖。将鉴定所得肽 通过使用LC/MS分析其Asn至Asp转化。所有结果将提交至生物信息学模块 (Core（4）与其他数据的整合。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core 2A is developing tools to analyze the Glycoprotein Glycome of cells, including embryonic mouse stem cells and their differentiated products. Fractionation of tryptic glycopeptides using serial lectin affinity chromatography is being developed to classify expression by the types of glycans expressed. Separation of glycopeptides is then further accomplished by liquid chromatography, followed by mass spectrometry to catalog and identify various species. At various stages, N-linked glycans will be produced by N-glycanase treatment. The resulting peptides will be identified by their Asn to Asp conversion using LC/MS analysis. All results will be submitted to the Bioinformatics module (Core project 4) for integration with other data.