IBR TRANSGENIC ANIMAL CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 转基因小鼠核心将利用生物发生研究所(IBR)教职员工的专业知识来开发核心设施，以增强夏威夷大学马诺阿分校(UH Manoa)的机构能力。我们将通过使用IBR开发的技术来创造最先进的转基因小鼠核心(TGMC)。五名初级调查人员中的四名，Alarcon博士、Moisyadi博士、M.Ward博士和Yamazaki博士已经掌握了IBR由柳崎龙藏博士开发的小鼠转基因所需的技术。最近，初级研究员Stefan Moisyadi博士开发了几种ICSI-Tr的修改，使用转座酶来提高转基因技术的效率。这将是莫伊西亚迪博士的第五个项目的重点。最后，初级研究员山崎骏博士非常擅长培养和操作小鼠胚胎干细胞，这种干细胞对生产所谓的“基因敲除小鼠”非常关键，在这种小鼠中，基因是从小鼠身上移除的，而不是添加的。开发转基因小鼠核心的理由是基于UH-M初级研究人员和其他研究人员目前的需求NOA和IBR中已经存在的独特专业知识。转基因小鼠核心将是一种独特的机制，可以增强整个夏威夷大学的机构能力，同时为IBR Cobre的初级研究人员提供重要资源。此外，IBR包含了UHM中唯一的一组科学家NOA，甚至在夏威夷州，他们拥有开发这样一个核心的专业知识。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Transgenic Mouse Core will draw upon the expertise of Institute for Biogenesis Research (IBR) faculty members to develop a core facility that augments institutional capacity at the University of Hawai`i at Manoa (UH Manoa). We will create a state-of-the-art Transgenic Mouse Core (TGMC) through using technology developed at the IBR. Four of the five junior investigators, Drs. Alarcon, Moisyadi, M. Ward, and Yamazaki, have mastered the techniques required for mouse transgenesis that were developed at the IBR by Dr. Ryuzo Yanagimachi. More recently, junior investigator Dr. Stefan Moisyadi has developed several modifications of ICSI-Tr that employ transposases to increase the efficiency of transgenic techniques. This will be the focus of Dr. Moisyadi's Project 5. Finally, junior investigator, Dr. Yamazaki, is very proficient in growing and manipulating mouse embryonic stem cells, which are critical to the production of so-called "knock-out mice" in which a gene is removed from the mouse rather than added. The rationale for developing the Transgenic Mouse Core is based on the current needs of both the junior investigators and other researchers at UH-M
noa and on the unique expertise that already exists in the IBR. A Transgenic Mouse Core would be a unique mechanism to enhance the institutional capacity of the entire University of Hawaii while providing an important resource to the junior investigators of the IBR COBRE. Moreover, the IBR contains the only group of scientists within UHM
noa and indeed in the State of Hawai`i, who have the expertise to develop such a core.