ROTAVIRUSES HAVE DIVERGENT GENE CONSTELLATIONS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 虽然分离的猿猴轮状病毒(RV)很少，但这些毒株对基础研究和疫苗开发都很重要。为了探索猴轮状病毒的起源，测定了PTRV(G8P[1])、RRV(G3P[3])和Tuch(G3P[24])株的全基因组序列。这些数据可以确定每种病毒的基因群，并阐明猿猴病毒株之间以及与非猿猴RV株之间的系统发育关系。结果表明，PTRV很可能是从牛或其他反刍动物传播到其起源宿主(猪尾猕猴)的，因为它的基因具有与牛RV相似的基因类型和编码外壳蛋白。相比之下，猕猴RRV和TUCH的大多数基因具有更典型的犬猫RV基因类型。然而，RRV和TUCH的犬/猫样基因序列仅与现代犬/猫RV的序列有较远的亲缘关系，这表明祖先病毒从犬/猫宿主传播到猿猴宿主并不是最近的事情。RRV和TUCH的其余基因似乎是通过与牛、人或其他RV毒株的重组而产生的。最后，将PTRV、RRV和TUCH基因与猕猴RV SA11-H96(G3P[2])的基因进行比较，表明SA11-H96与其他猿猴株的遗传相似性很小，可能是独立进化的。总体而言，我们的数据表明，猿类房车具有不同的祖先，其基因组星座主要起源于物种间的传播和与非人类动物房车的重新分类。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Although few simian rotaviruses (RVs) have been isolated, such strains have been important for basic research and vaccine development. To explore the origins of simian RVs, the complete genome sequences of strains PTRV (G8P[1]), RRV (G3P[3]), and TUCH (G3P[24]) were determined. This data allowed the genotype constellations of each virus to be determined and the phylogenetic relationships of the simian strains with each other and with non-simian RVs to be elucidated. The results indicate that PTRV was likely transmitted from a bovine or other ruminant into its host of origin (pig-tailed macaques), as its genes have genotypes and encode outer-capsid proteins similar to those of bovine RVs. In contrast, most of the genes of rhesus-macaque strains, RRV and TUCH, have genotypes more typical of canine-feline RVs. However, the sequences of the canine/feline-like genes of RRV and TUCH are only distantly-related to those of modern canine/feline RVs, indicating that transmission of the progenitor virus from a canine/feline host to a simian host was not recent. The remaining genes of RRV and TUCH appear to have originated through reassortment with bovine, human, or other RV strains. Finally, comparison of PTRV, RRV, and TUCH genes with those of the vervet-monkey RV SA11-H96 (G3P[2]) indicates that SA11-H96 shares little genetic similarity to other simian strains and likely has evolved independently. Collectively, our data indicate that simian RVs are of diverse ancestry with genome constellations that originated largely by interspecies transmission and reassortment with non-human animal RVs.