NON-HUMAN PRIMATE PROPAGATION BY SOMATIC CELL NUCLEAR TRANSFER

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目的主要目标是了解核移植的基本生物学，最终目标是加深对胚胎干细胞的了解，并可能产生克隆的非人类灵长类后代。 为了实现这一目标，将从狒狒身上收集卵母细胞，然后在实验室以各种方式进行操纵，以产生克隆和/或控制胚胎。这些胚胎中的大部分将保留在实验室中，用于体外研究。然而，为了确定怀孕，一部分胚胎将被移植到接受移植的雌性体内。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. There major goal of this project is to understand the underlying biology of nuclear transfer, with the ultimate goal of furthering the understanding of embryonic stem cells and to perhaps produce a cloned non-human primate offspring. In order to achieve this, oocytes will be collected from baboons and then manipulated in various ways in the lab to produce cloned and/or control embryos. The majority of these embryos will be retained in the laboratory for use in in vitro studies. However, a portion of the embryos will be transferred into recipient females in order to establish pregnancies.