ADVANCED TECH FOR PROCESSING & ANALYSIS OF MULTI-RESOLUTION, LIGHT AND EM DATA

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 用于多分辨率、光和电子显微镜数据处理和分析的高级逻辑 这项技术研究和开发活动的目的是为NCMIR研究人员提供专门针对涉及大量多模态3D和4D数据的中尺度调查的重建，可视化和分析工具。 我们将继续遵循成功的战略，在可能的情况下利用社区工具，并与图像分析和可视化领域的领导者合作。 我们将自己的开发工作用于在必要时增强这些工具，并围绕NCMR和我们的合作者生成的数据类型开发新的分析方法。 具体工作将涉及新的成像模式和标本几何形状的重建算法的增强;电子显微镜数据的半自动和手动分割的工具和方法的改进;利用电子显微镜数据通知光显微镜分析;和大型，多维数据集的可视化工具和平台的开发。 如有需要，我们将与工具供应商合作，调整其工具，以处理通过核心项目4A2.1和4A2.2中描述的新成像模式和仪器产生的大量数据集。 由此产生的产品将被集成到NCMIR的先进网络基础设施中，并将专门设计为各种工具和成像工作流程提供集成环境，这将提高我们管理大型复杂多尺度数据集的能力。 每项活动也将与核心项目4A3.2所述的我们已建立的信息学基础设施保持一致。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. ADVANCED TECHNOLOGIES FOR THE PROCESSING AND ANALYSIS OF MULTI-RESOLUTION, LIGHT AND ELECTRON MICROSCOPY DATA The aim of this technological research and development activity is to provide NCMIR researchers with an arsenal of reconstruction, visualization, and analysis tools specifically tailored to mesoscale investigations involving massive multi-modal 3D and 4D data. We will continue to follow a successful strategy of utilizing community tools where possible and partnering with leaders in image analysis and visualization. We direct our own development efforts to augmenting these tools where necessary and developing novel analysis approaches around the types of data generated by NCMIR and our collaborators. Specific work will involve enhancement of reconstruction algorithms for new imaging modes and specimen geometries; improvement of tools and approaches for semi-automated and manual segmentation of electron microscopic data; utilization of electron microscopic data to inform light microscopic analyses; and development of tools and platforms for visualization of large, multidimensional data sets. Where necessary, we will work with tool providers to adapt their tools to handle the massive data sets generated through new imaging modes and instrumentation described in core projects 4A2.1 and 4A2.2. The resulting products will be integrated within NCMIR's advanced cyberinfrastructure, and will be specifically designed to provide an integrated environment for diverse tools and imaging workflows that will improve our ability to manage large and complex multi-scale datasets. Each activity will also be brought into alignment with our established informatics infrastructure to be described in core project 4A3.2.