NMR STUDY OF SMALL HEAT SHOCK PROTEIN (HSP12) FROM CESG

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 CESGHSP12中小分子热休克未折叠蛋白(HSP12)的核磁共振研究是一种小分子热休克蛋白，能保护细胞膜免受脱水，在常规的核磁共振缓冲液中可以解折叠。初步研究表明，在洗涤剂(十二烷基硫酸钠)存在下，这种蛋白质可以折叠。我们计划在十二烷基硫酸钠存在的情况下确定结构。此外，我们正在使用HiFi快速数据方法对蛋白质进行分配。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NMR study of small heat shock unfolded protein (HSP12) from CESGHSP12 is a small heat shock protein, which protects the membrane from dehydration and is unfolded in regular NMR buffer. Priliminary studies indicate this protein folds in presence of detergents (SDS). we are planning to determine the structure in presence of SDS. In addition we are using HIFI fast data method to assign the protein.