MICRO FLUORESCENCE DEVELOPMENT

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们改进了荧光微探针装置的性能。 系统现在能够以每点0.2秒的速度扫描样品。这种快速扫描功能可以将每个样品的总扫描时间减少到30分钟。 我们实现了一个双光谱仪系统连接到我们现有的单元素硅漂移探测器。 这种设置允许使用单个光谱仪最小化连续扫描的开销时间。 显微荧光-显微衍射装置可以在大约30分钟内更换，显著减少了切换实验装置的停机时间。 MAPS分析软件已经在我们的光束线上实现。该软件被广泛用于其他光束线分析荧光映射数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have improved the performance of the fluorescence microprobe setup. The system is now able to scan samples as fast as 0.2 seconds per point. This fast scan capability allows reducing the total scanning time per sample down to 30 minutes. We implemented a dual spectrometer system connected to our existing single element silicon drift detector. This setup allows minimizing the overhead time of the continuous scans using a single spectrometer. The microfluorescence- microdiffraction setups can be exchanged in about 30 minutes reducing significatively the down time to switch experimental arrangements. MAPS analysis software has been implemented in our beamline. This software is widely used at other beamlines to analyze fluorescence mapping data.