MICROARRAY VALIDATION OF DATA FROM WILD-TYPE AND MGAT5 KNOCK-OUT MOUSE TISSUES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 通过对野生型和mGat5基因敲除小鼠组织进行微阵列分析，确定转录水平增加或减少的基因正在用qRT-PCR作为验证工具进行分析。野生型和mGat5基因敲除的小鼠组织中的一组原钙粘附素基因的转录水平也在测定中。原钙粘附素是较大的钙粘附素超家族中的一个亚家族，被认为与细胞黏附有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Genes that were determined to be increased or decreased in transcript level by microarray analysis of wild type and Mgat5 knock-out mouse tissues are being analyzed by qRT-PCR as a validation tool. Transcript levels are also being determined for a group of protocadherin genes from wild type and Mgat5 knock-out mouse tissues. Protocadherins comprise a subfamily within the larger cadherin superfamily and are thought to be involved in cell adhesion.