Project 4: Measuring Chronic Exposure To And Bioavailability Of Organic Chemicals

拟议的研究涉及检测研究的广泛SRP主题，更具体地说，是开发用于多种化学物质检测和测定水和沉积物中生物利用度的被动采样器。目标1将开发一种通用被动采样装置（PSD），用于测量水中数百种有机化学物质的时间加权平均慢性暴露。我们将推进PSD的理论、设计和应用到非常广泛的物理化学性质（例如，KOW = 0-9），以便几乎所有超级基金优先化学品清单上的有机化学品及其许多代谢物都将被单个PSD采样。我们假设混合聚合物吸附相包含在非选择性和高度多孔膜将允许线性吸收几乎所有的有机化学物质。目标2将建立psd的使用，以测量水、沉积物和土壤中多氯联苯和多氯联苯代谢物的生物可利用部分。我们将对多氯联苯污染的土壤、沉积物和水进行实验室生物利用度实验，以加深我们对多氯联苯生物利用度控制机制的理解，并在国家物理实验室进行实地验证。在与项目1的合作中，我们将使用样品的提取物来确定我们的生物利用度测量与项目1细胞测定中二恶英毒性等效因子反应的关系。目标3将建立psd的使用，以测量水、沉积物和土壤中多环芳烃和多环芳烃代谢物的生物可利用部分。与项目5合作，我们将进行与目标2非常相似的研究，以开发psd在受控实验室条件和国家物理实验室站点测量多环芳烃和多环芳烃代谢物生物利用度的使用。这项工作将

The proposed research addresses the broad SRP theme of Detection Research and more specifically the development of passive samplers for multi-chemical detection and determination of the degree of bioavailability in water and sediment. Aim 1 will develop a universal passive sampling device (PSD) for measuring the time-weighted-average chronic exposure to hundreds of organic chemicals in water. We will advance the theory, design, and application of PSDs to a very broad range of physico-chemical properties (e.g., KOW = 0-9) so that nearly every organic chemical on the Superfund Priority List of Chemicals, and many of their metabolites, will be sampled by a single PSD. We hypothesize that a mixed-polymer sorptive phase contained within a non-selective and highly porous membrane will allow linear uptake of nearly all organic chemicals. Aim 2 will establish the use of PSDs to measure the bioavailable fraction of PCBs and PCB metabolites in water, sediment, and soil. We will conduct laboratory bioavailability experiments with PCB-contaminated soil, sediment and water to advance our understanding of the mechanisms controlling PCB bioavailability and perform field verification at NPL sites. In collaboration with Project 1, we will use extracts of our samples to determine the relationship of our bioavailability measure to the dioxin toxic equivalency factor response in Project 1 cell assays. Aim 3 will establish the use of PSDs to measure the bioavailable fraction of PAHs and PAH metabolites in water, sediment, and soil. In collaboration with Project 5, we will perform studies very similar to those in Aim 2 to develop the use of PSDs to measure PAH and PAH metabolite bioavailability under both controlled laboratory conditions and at NPL sites. This work will advance our understanding of and ability to measure the partitioning of PAHs and metabolites among dissolved organic carbon, soft and hard (soot) particulate carbon, weathered oil/oil product phases, and biota. We hypothesize that our novel PSD design will provide an accurate measure of bioavailable chronic exposure under a broad range of conditions and that PSD-derived data will overcome a critical barrier to more accurate estimates of bioavailability and risk.