Agilent 6490 LCMS Triple Quadrupole Mass Spectrometer with 1260 Infinity Chip Cub

描述（由申请人提供）：我们正在申请资金购买一个多功能的，高灵敏度的高通量系统，包括一个HPLC芯片接口的三重四极杆质谱仪。该仪器将位于查佩尔山（UNC-CH）的北卡罗来纳州大学环境科学与工程系的生物标志物质谱设施核心，这是一个共享设施，为整个大学及其他地区的研究人员提供服务。这种仪器将为我们提供比现有仪器至少高一个数量级的灵敏度。这将允许检测和定量分析物，这些分析物对于我们目前的仪器来说水平太低，和/或可用样品的总量排除了我们目前仪器的分析。选择的设备是通过1260 Infinity Chip Cube与Agilent 6490 LCMS三重四极杆质谱系统连接的Agilent 1200系列纳米流HPLC。该组合是市场上最灵敏的LC-MS/MS，并且将允许以最小的样品质量和体积定量感兴趣的生物标志物。Chip Cube接口也非常适合在共享仪器设施中使用，在这些设施中，痕量级分析很容易受到交叉污染的影响。这些设备将支持9名NIH资助的研究人员。 公共卫生相关性：生物标志物质谱设施为位于查佩尔山的北卡罗来纳州大学生物医学研究社区提供测量小分子和生物标志物的可访问常规服务。生物标志物质谱设施所需的LCMS/MS系统将使该设施能够将复杂生物基质中小分子、DNA加合物、DNA-蛋白质交联和其他生物标志物的检测灵敏度至少提高一个数量级。这将加强UNC-CH公共卫生学院和医学院的九名NIH资助的研究人员的研究，其中包括三名医生和科学家，他们解决与癌症，囊性纤维化和其他呼吸系统疾病和药物滥用有关的关键健康问题。

DESCRIPTION (provided by applicant): We are requesting funding to purchase a versatile, high sensitivity high-throughput system consisting of an HPLC-chip interfaced to a triple quadrupole mass spectrometer. This instrument will be located in the Biomarker Mass Spectrometry Facility Core in the Department of Environmental Sciences and Engineering of the University of North Carolina at Chapel Hill (UNC-CH), which is a shared facility that serves researchers from the entire university and beyond. This instrumentation will provide us with at least an order of magnitude higher sensitivity than the existing instrumentation. This will allow detection and quantitation of analytes that are present at levels too low for our current instrumentation, and/or where the total amount of sample available precludes analysis with our current instrumentation. The equipment of choice is an Agilent 1200 series nanoflow HPLC interfaced via a 1260 Infinity Chip Cube with an Agilent 6490 LCMS Triple Quadupole mass spectrometry system. This combination is the most sensitive LC-MS/MS on the market, and will allow quantitation of biomarkers of interest with the smallest mass and volume of sample. The Chip Cube interface is also very well suited for use in a shared instrument facility where trace-level analysis is very easily compromised by cross-contamination. This equipment will support nine NIH- funded researchers. PUBLIC HEALTH RELEVANCE: The Biomarker Mass Spectrometry Facility provides accessible routine service for measuring small molecules and biomarkers to the University of North Carolina at Chapel Hill biomedical research community. The LCMS/MS system requested for the Biomarker Mass Spectrometry Facility will enable the Facility to extend by at least an order of magnitude the sensitivity of assays for small molecules, DNA adducts, DNA-protein crosslinks, and other biomarkers in complex biological matrices. This will enhance studies by nine NIH-funded researchers at the UNC-CH School of Public Health and School of Medicine, including three physicianscientists, who address critical health problems related to cancer, cystic fibrosis and other respiratory disease and drug abuse.