Agilent 6490 LCMS Triple Quadrupole Mass Spectrometer with 1260 Infinity Chip Cub

配备 1260 Infinity Chip Cub 的 Agilent 6490 LCMS 三重四极杆质谱仪

• 批准号: 8247540
• 负责人: JAMES A SWENBERG
• 金额: $ 39.7万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247540
• 关键词: Biological Markers; Core Facility; Detection; Ecology; Environmental Engineering technology; Equipment; Funding; High Pressure Liquid Chromatography; Marketing; Mass Spectrum Analysis; North Carolina; Research Personnel; Sampling; Series; System; United States National Institutes of Health; Universities; instrument; instrumentation; interest; mass spectrometer

DESCRIPTION (provided by applicant): We are requesting funding to purchase a versatile, high sensitivity high-throughput system consisting of an HPLC-chip interfaced to a triple quadrupole mass spectrometer. This instrument will be located in the Biomarker Mass Spectrometry Facility Core in the Department of Environmental Sciences and Engineering of the University of North Carolina at Chapel Hill (UNC-CH), which is a shared facility that serves researchers from the entire university and beyond. This instrumentation will provide us with at least an order of magnitude higher sensitivity than the existing instrumentation. This will allow detection and quantitation of analytes that are present at levels too low for our current instrumentation, and/or where the total amount of sample available precludes analysis with our current instrumentation. The equipment of choice is an Agilent 1200 series nanoflow HPLC interfaced via a 1260 Infinity Chip Cube with an Agilent 6490 LCMS Triple Quadupole mass spectrometry system. This combination is the most sensitive LC-MS/MS on the market, and will allow quantitation of biomarkers of interest with the smallest mass and volume of sample. The Chip Cube interface is also very well suited for use in a shared instrument facility where trace-level analysis is very easily compromised by cross-contamination. This equipment will support nine NIH- funded researchers. PUBLIC HEALTH RELEVANCE: The Biomarker Mass Spectrometry Facility provides accessible routine service for measuring small molecules and biomarkers to the University of North Carolina at Chapel Hill biomedical research community. The LCMS/MS system requested for the Biomarker Mass Spectrometry Facility will enable the Facility to extend by at least an order of magnitude the sensitivity of assays for small molecules, DNA adducts, DNA-protein crosslinks, and other biomarkers in complex biological matrices. This will enhance studies by nine NIH-funded researchers at the UNC-CH School of Public Health and School of Medicine, including three physicianscientists, who address critical health problems related to cancer, cystic fibrosis and other respiratory disease and drug abuse.

描述（由申请人提供）：我们正在申请资金购买多功能、高灵敏度高通量系统，该系统由连接到三重四极杆质谱仪的 HPLC 芯片组成。该仪器将位于北卡罗来纳大学教堂山分校 (UNC-CH) 环境科学与工程系 (UNC-CH) 的生物标志物质谱设施核心内，这是一个为整个大学内外的研究人员提供服务的共享设施。该仪器将为我们提供比现有仪器至少高一个数量级的灵敏度。这将允许检测和定量分析物，这些分析物的含量对于我们当前的仪器而言太低，和/或可用样品的总量妨碍了我们当前仪器的分析。选择的设备是 Agilent 1200 系列纳流 HPLC，通过 1260 Infinity Chip Cube 与 Agilent 6490 LCMS 三重四极杆质谱系统连接。该组合是市场上最灵敏的 LC-MS/MS，并且可以使用最小质量和体积的样品对感兴趣的生物标志物进行定量。 Chip Cube 接口也非常适合在共享仪器设施中使用，在这种设施中，痕量级分析很容易受到交叉污染的影响。该设备将为 NIH 资助的九名研究人员提供支持。 公共健康相关性：生物标志物质谱设施为北卡罗来纳大学教堂山分校生物医学研究界提供测量小分子和生物标志物的常规服务。生物标志物质谱设施所需的 LCMS/MS 系统将使该设施能够将小分子、DNA 加合物、DNA-蛋白质交联和复杂生物基质中其他生物标志物的检测灵敏度提高至少一个数量级。这将加强 UNC-CH 公共卫生学院和医学院的九名 NIH 资助的研究人员的研究，其中包括三名医师科学家，他们解决与癌症、囊性纤维化和其他呼吸道疾病和药物滥用相关的关键健康问题。