Rocky Mountain Neurological Disorders Core

这是落基山神经紊乱中心(RMNOC)的竞争性更新，其主要目标是 是加强和进一步研究尼诺斯人瞄准的神经生物学领域。RMNO中心核心 已经建立了设施，允许高效地产生和使用转基因动物 有趣且信息量大的功能或行为表型。我们现有的核心都是非常 成功并发展为支持最先进的方法，从而允许用户执行协作 以高效的方式进行研究。因此，我们建议保留原有的核心名称， 强调它们将在接下来的五年中继续发展，如申请中所述(核心A， 6、C和0)。和以前一样，安舒茨的其他地方没有四个核心提供的服务 医学院。 我们建议为RMNO中心核心提供四项核心设施： 核心A显微镜核心(比尔·贝茨，董事)将提供先进的光学和电子显微镜服务。 核心B基因打靶/病毒载体核心(Mark Dell‘Acqua，董事)\N\设计和构建载体以 产生基因靶向和转基因小鼠以及病毒靶向载体。 核心CZebraFish转基因核心(洛杉矶，里贝拉，董事)m\\提供维护和 转基因斑马鱼的特征。 CORE D机械车间核心(Ulli Bayer，总监)将提供机械车间支持服务。

This Is a competitive renewal of the Rocky Mountain Neurological Disorder Center (RMNOC) whose main goal Is to enhance and further research in areas of neurobiology targeted by the NINOS. The RMNO Center Core has established facilities that allow efficient generation and use of genetically-altered animals that display Interesting and Informative functional or behavioral phenotypes. Our existing cores have all been extremely successful and evolved to enable state-of-the-art approaches thereby allowing users to perform collaborative research in an efficient manner. Therefore, we propose to maintain the original cores with their original names, emphasizing that they will continue to evolve over the next five years, as described in the application (Cores A, 6, C, and 0). As before, the services provided by the four cores are not available elsewhere at the Anschutz Medical Campus. We propose four core facilities for the RMNO Center Core: Core A Microscopy Core (Bill Betz, Director) will provide advanced light and electron microscopy services. Core B Gene-Targeting/Viral Vector Core (Mark Dell'Acqua, Director) \N\\\ design and construct vectors to generate gene-targeted and transgenic mice as well as viral targeting vectors. Core CZebrafish Transgenic Core (Angeles Ribera, Director)m\\ provide services for maintaining and characterizing transgenic zebraflsh. Core D Machine Shop Core (Ulli Bayer, Director) will provide machine shop support services.