Analytical Cytochemistry

分析细胞学核心(ACC)的目标是提供先进的设备和经验丰富的操作员来测量和分离细胞及其组件的属性，并提供数据供内部分析和外部审查。这一核心资源中的流式细胞仪仪器包括(1)高速细胞分选仪(MoFlo)和(2)分析细胞仪(Cyan和FacsCalibur)。流式细胞仪为研究人员提供了分析和分离细胞的工具，分析和分离细胞的速度高达35,000个/秒，基于多个荧光标记和光散射特性，产量高(基于速度高达90%) 和极高的纯度(99%)。由于能够一次根据多个参数对四个种群进行分类，大大减少了调查人员的后勤和成本。一种新的用于青色分析器的高通量平板采样器为拥有大型化合物文库的研究人员提供了快速筛选能力，他们使用流式细胞仪进行化合物鉴定；激光扫描细胞仪(LSC)的增加扩大了ACC提供的分析能力，包括基于定量组织的分析能力 荧光显微镜。该仪器同时收集列表模式荧光数据和显微图像，并为用户提供将大型组织切片上的定量荧光数据与定性显微图像相关联的能力。LSC由经过专门培训的用户运行，并由专门的操作员管理。行政协调会仪器每周进行质量控制评估，并进行例行预防性维护和校准。核心中产生的数据可通过BRI-Net服务器获得，以供进一步分析和准备提交或出版。核心提供基于网络的数据处理软件，正在开发实验室信息管理系统(LIMS)，以帮助跟踪与实验有关的元数据和档案检索。在2006财年，来自所有5个项目的44名癌症中心成员和3名非联合成员使用了ACC(的同行评议使用率)。这一核心的年度预算为401,799美元(43%的机构费用，40%的使用费)；17%(70100美元)由协调委员会申请。

The goal of the Analytical Cytometry Core (ACC) is to provide leading-edge equipment and experienced operators to measure properties of, and isolate, cells and their components, and present the data for internal analysis and external review. Flow cytometry instrumentation in this core resource includes (1) high speed cell sorter (MoFlo) and (2) analytical cytometers (Cyan and FacsCalibur). Flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 35,000 cells/second based on multiple fluorescent labels and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Owing to the capability to sort for four populations at a time on multiple parameters, the logistics and cost for the investigators are substantially reduced. A new high throughput plate sampler for the Cyan analyzer provides rapid screening capacity to investigators with large compound libraries who use flow cytometric analysis in compound identification; the addition of a Laser Scanning Cytometer (LSC) has expanded the analytical capacity offered by the ACC to include quantitative tissuebased fluorescence microscopy. This instrumentation collects both listmode fluorescence data and microscopic images and provides users with the capacity to correlate quantitative fluorescent data with qualitative microscopic images on large tissue sections. The LSC is run by specially trained users and managed by a dedicated operator. ACC instrumentation is subject to weekly quality control assessment and routine preventive maintenance and calibration. Data generated in the Core is available through the BRI-net server for further analysis and preparation for presentation or publication. Network-based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment-related meta data and archived file retrieval. During FY 2006, the ACC was used by 44 Cancer Center members from all 5 programs and 3 non-aligned members (64% peer-reviewed usage). Annual budget for this core is $401,799 (43% institutional, 40% user fees); 17% ($70,100) is requested from the CCSG.