Immunologic Monitoring

免疫监测核心为密歇根大学癌症中心和生物医学界正在进行的研究项目和临床试验提供免疫和生物分析支持。在三个不同的领域提供服务。首先，为研究者持续提供大量细胞因子、趋化因子、激素等释放蛋白的同时检测及相关技术支持。这些检测使用Luminex[200]系统进行，并使用机器人移液站通过ELISA进行。其次，taa特异性T细胞反应分析将提供给研究人员。这些检测包括taa特异性细胞因子ELISA斑点检测（AID ELISPOT Plate Reader）， taa特异性T细胞增殖和CTL活性检测。第三，核心为没有标准湿实验室空间的研究人员提供时间标准技术服务，以支持免疫生物学领域的临床研究方案。该设施将提供对组织进行处理和分析的机会，用于一般免疫参数，标准淋巴细胞和肿瘤增殖测定，以及细胞因子的定量。在非免疫学实验室中，这些免疫检测通常无法获得，the Core还提供专家建议，帮助研究人员使用合适的试剂选择合适的免疫检测，并将其应用于最佳优势。

The Immunological Monitoring Core provides immunologic and biological assay support for ongoing research projects and clinical trials at the Cancer Center and the biomedical community at the University of Michigan. Services are provided in three different areas. First, simultaneous detection of numerous cytokines, chemokines, hormones and other released proteins and the related technical support are provided on a continued basis for investigators. These assays are performed with the Luminex[200] System, and via ELISA using a robotics pipetting station. Second, TAA-specific T cell response assays will be provided to investigators. These assays Include TAA-specific cytokine ELISA spot by using the AID ELISPOT Plate Reader, and TAA-specific T cell proliferation and CTL activities. Third, the Core provides time standard technical services to support clinical research protocols in the immunobiology area for Investigators who do not have standard wet laboratory space. This facility will provide the opportunity to have tissues processed and analyzed for general immune parameters, standard lymphocyte and tumor proliferation assays, as well as quantification of cytokines. These Immune assays may not nomially be available In the non-immunology laboratory, The Core also provides expert advice that helps investigators select appropriate immune assays using appropriate reagents and apply them to best advantage.