Comparative Pathology Core

项目总结(见说明)： 比较病理学核心A将为三个科学项目提供集中的病理学支持，并代表着从最初的赠款周期到小鼠病理生物学核心的演变。比较病理学核心的一个主要目标将是提供在小鼠和人类病理学方面的专业知识，以及对乳腺和乳腺癌病理学的最新熟练程度。此外，在教授病理学家的监督下，一名小鼠病理学博士和博士后将进行小鼠身体解剖、组织切割和处理，以及组织病理学和特殊染色的评估。这将确保为研究项目提供卓越和持续的服务。核心将有6名人员，其中包括3名 病理学家(一名内科和两名兽医)、内科肿瘤学家(乳腺癌专家)、一名病理研究员和兼职组织技术员。比较病理学核心对于确保将小鼠遗传模型转化为人类乳腺癌的特定目标和实验的整合至关重要。这三位病理学家和肿瘤学家在合作项目上有着长期的合作记录，因此可以很好地为Core A提供支持。此外，这三位病理学家和肿瘤学家都与奥斯托夫斯基博士和里昂博士合作，共同撰写了对乳腺癌微环境及其与乳腺癌进展的关系具有高度影响的手稿(1，2)。设施将包括四个尸检室(包括一个专门研究老鼠病理的房间)、组织处理设施、有四名全职技术人员(一名专门研究核心)的组织学实验室和一台自动达科免疫染色机。具有Ventana染色系统和Nuance plus的原位分子病理学核心实验室基于计算机对分子靶标的共表达进行分析， 显微镜设施包括一台可为会议输出视频的10头显微镜、激光捕获显微解剖设备和工作人员、组织微阵列实验室、小鼠成像设备(包括高分辨率超声波、数字大体和显微摄影)、组织形态测量设备 荧光显微镜和Image Pro Plus分析软件，以及鼠标手术设施。建立一个SHAREPOINT网站将便利核心和项目工作人员之间的数据传输和共享。这些教师和服务将为研究项目提供独特的资源，并显著提高研究数据的质量和有效性。

PROJECT SUMMARY (See instructions): The Comparative Pathology Core A will provide centralized pathology support for the three scientific projects and represents an evolution from the Mouse Pathobiology Core from the initial grant cycle. A major goal of the Comparative Pathology Core will be to provide expertise in mouse and human pathology with up-to-date proficiency in mammary gland and breast cancer pathology. In addition, a DVM, PhD postdoc in mouse pathology will perform the mouse necropsies (autopsies), cutting and processing of tissues, and evaluation of histopathology and special stains under the supervision of faculty pathologists. This will ensure excellence and continuity of service for the research projects. The Core will have 6 personnel that include three pathologists (one medical and two veterinary), medical oncologist (breast cancer specialist), a pathology fellow, and part-time histology technician. The Comparative Pathology Core is essential to ensure integration of the specific aims and experiments in relation to translation of the mouse genetic models to human breast cancer. The three pathologists and oncologist have a long track record of working together on collaborative projects, and therefore are well positioned to provide support for Core A. In addition, all three pathologists and the oncologist have collaborated with Drs. Ostrowski and Leone leading to shared authorship on manuscripts with high impact on mammary cancer microenvironment and its relationship to mammary cancer progression (1, 2). Facilities will include four necropsy rooms (including one devoted to mouse pathology), tissue processing facilities, histology laboratory with four full-time technicians (one devoted to the Core) and an automated Dako immunostainer. In Situ Molecular Pathology Core Lab with a Ventana staining system and NUANCE PLUS InForm computer-based analyses of co-expression of molecular targets, microscopy facilities including a 10-headed microscope with video output for conferences, laser capture microdissection facility and staff, tissue microarray laboratory, mouse imaging facilities including high resolution ultrasound, digital gross and microscopic photography, histomorphometry equipment including a fluorescent microscope and Image Pro Plus analysis software, and mouse surgery facilities. A SharePoint website will facilitate data transfer and sharing between the cores and project staff. These faculty and services will provide a unique resource to the research projects and significantly enhance the quality and validity of the research data.