Project 2: Development of Rapid, Miniaturized Biosensors

生物传感器项目的目标是应用新的和新兴的技术来实施我们在Davis的同事开发的生物检测，与传统方法相比，在速度和/或灵敏度方面有所改进。将测试几种新的检测方案，包括用于捕获和询问用于DNA和免疫分析的非漂白纳米颗粒标签的纳米孔格式；用于DNA分析的磁性/发光纳米颗粒格式；以及用于增强荧光检测的纳米结构液芯波导。目标将包括拟除虫菊酯代谢物3-PBA、新出现的问题化合物，如TCC、TCS和PBDE，以及用于生物修复活动的微生物基因。将基于AhR技术开发TCDD及其相关化合物的传感器。

It is the goal of this project Biosensors, to apply new and emerging technologies to implement bioassays that our colleagues at Davis have developed, with improvements in speed and/or sensitivity compared to conventional methods. Several new schemes for detection will be tested, including a nanowell format for trapping and interrogating our non-bleaching nanoparticle labels for DNA and immune assays; a magnetic/luminescent nanoparticle format for DNA assays; and a nanostructured liquid core waveguide for enhancement of fluorescence detection. The targets will include the pyrethroid metabolite 3-PBA, emerging problem compounds such as TCC, TCS and PBDE, and genes for microbes that are used in bioremediation activities. A sensor for TCDD and related compounds will be developed based on AhR technology.