Basic Science Shared Resource Group

项目总结（见说明）： 显微镜和细胞分析中心设备齐全，工作人员专业，为来自临床和基础科学实验室的马约诊所癌症中心研究人员提供标本制备、光学和电子显微镜、摄影、数字成像以及多参数流式细胞和细胞分选服务。该设施还进行X射线探针显微分析，免疫金标记，3-D EM重建从连续切片标本，并使用图像平均负染色和冷冻断层扫描成像标本的高分辨率重建。仪器和专业知识也支持高端光学，双光子，超分辨率，激光共聚焦，TIRF，比率成像和其他光学方法，以及流式细胞术和活细胞分选技术和分析的核心。此外，核心工作人员（23 FTE）和核心主任可用于开发和/或实施新的显微镜技术，并测试和评估仪器，这将是有价值的个人马约癌症中心的研究人员和更大的研究社区。在上一个预算年度，显微镜和细胞分析共享资源已被马约癌症中心的每个项目广泛使用。在过去的12个月里，该设施为100多名马约癌症中心的研究人员提供服务，处理了1000多个电子显微镜标本，记录了5000小时的光学显微镜检查和5000多小时的流式分析和细胞分选。由于高端光学和电子显微镜的购买和维护费用高昂，以及其生产使用所需的专业知识，大多数研究人员及其研究实验室发现显微镜和细胞分析核心服务是最经济，实用和最新的手段，以实现其复杂的显微镜和细胞分选和分析研究需求。

PROJECT SUMMARY (See instructions): The Microscopy and Cell Analysis Core is fully equipped and expertly staffed to perform specimen preparation, light and electron microscopy, photography, digital imaging, and multi-parameter Flow and Cell Sorting services to Mayo Clinic Cancer Center investigators from both clinical and basic science laboratories. The facility also performs X-ray probe microanalysis, immuno-gold labeling, 3-D EM reconstruction from serial-sectioned specimens, and high-resolution reconstruction using image averaging of negative stained and cryo-tomography imaged specimens. Instrumentation and expertise are also supported in the core for high-end optical, two-photon, super-resolution, laser confocal, TIRF, ratio imaging, and other optical methods, as well as Flow Cytometry and Live Cell Sorting technologies and analysis. Additionally, the core staff (23 FTEs) and the core director are available for the development and/or implementation of new microscopy techniques and for testing and evaluation of instrumentation that would be of value to individual Mayo Cancer Center investigators and the greater research community. During the last budget year, the Microscopy and Cell Analysis Shared Resource has been widely utilized by each of the Mayo Cancer Center Programs. During the past 12 months, the facility served over 100 Mayo Cancer Center investigators processing 1000-plus specimens for electron microscopy, and logging 5,000 hours of Optical Microscopy and over 5000 hours of Flow Analysis and Cell Sorting. Because of the large expense for purchase and maintenance of high-end optical and electron microscopes, and the expertise required for their productive use, most investigators and their research laboratories find the Microscopy and Cell Analysis Core services to be the most economical, practical, and up-to-date means to achieve their sophisticated microscopy and cell sorting and analysis research needs.