Acquisition of Sony Biotechnology iCyt Synergy SY3200 BSC Cell Sorter

描述(由申请人提供)：来自约翰霍普金斯大学的13名研究人员，包括艺术与科学学院(和生物系)；怀廷工程学院(以及化学和生物分子工程系以及肿瘤学中心的PS-OC工程系)；以及联合纪念医院和整形生物实验室申请519,686美元购买索尼生物技术Synergy SY3200细胞分类器。该系统将安装在约翰·霍普金斯大学综合成像中心(IIC，：//www.jhu.edu/iic)；并成为升级后的BSL-2细胞分选设施的核心，服务于霍普金斯大学霍姆伍德校区和北巴尔的摩地区的不同需求。IIC是霍姆伍德校园/霍普金斯大学范围内的显微镜资源，由克里格艺术与科学学院和怀廷工程学院联合支持(参见Ball&Douglas Letter)；由&gt；150个实验室和&gt；450个用户组成的多个学校和部门定期使用。新的Synergy分选机将用DIVA选件取代BD FACSVantage SE，其过时的设计和复杂性使常规操作/使用变得困难和昂贵；在分选过程中出于生物遏制的考虑对操作员来说很危险，这必然将分选限制在BL-1级标本；由于其老化和缺乏部件可用，因此不可靠和难以维护。新的iCyt Synergy SY3200分拣机集成了一个分拣头，集成到Baker SterilGARD(R)11罩中，使其特别安全 操作员通过计算机远程影响分拣机的操作/调整。喷嘴交换是一种简单而必要的自动对准和计算机控制。此外，由于分拣机头集成到Baker BSC中，设计特别紧凑。安装后，新系统将提供北巴尔的摩和霍普金斯大学霍姆伍德校区目前无法提供的BSL-2分拣能力。目前，用户必须等待数周才能获得霍普金斯大学东巴尔的摩校区和湾景校区的分拣服务，这些校区分别位于5英里和10英里之外，非常不方便。它将被纳入IIC现有的、完善的充值系统，以确保收回用于供应和维护的资金；它将通过研讨会和动手培训向霍普金斯大学所有感兴趣的用户免费提供；它将被纳入生物技术教育中心的生物技术硕士课程；以及IIC的年度本科生/研究生课程。(：www.jhu.edu/iic/academic.htm)。

DESCRIPTION (provided by applicant): 13 investigators from The Johns Hopkins University, comprising the School of Arts & Sciences (and the Department of Biology); the Whiting School of Engineering (and the Department of Chemical and Biomolecular Engineering and the PS-OC Engineering in Oncology Center); and the Union Memorial Hospital and the Orthobiologic Laboratory request $519,686 to purchase a Sony Biotechnology Synergy SY3200 Cell sorter. This system will be sited in the Johns Hopkins' Integrated Imaging Center (IIC, ://www.jhu.edu/iic); and become the centerpiece of an upgraded BSL-2 cell sorting facility serving the diverse needs of the Hopkins' Homewood Campus and North Baltimore area generally. The IIC is a Homewood campus/Hopkins-wide microscopy resource, jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering (see Ball & Douglas letter); and utilized regularly by multiple schools and departments comprising >150 laboratories and >450 users. The new Synergy sorter will replace a BD FACSVantage SE with DIVA option whose antiquated design and complexity makes it difficult and costly to operate/use routinely; dangerous for the operator due to bio- containment concerns during sorting that necessarily limit sorting to BL-1 level specimens; and unreliable and problematic to maintain due to its age and lack of parts availability. The new iCyt Synergy SY3200 sorter incorporates a sorting head that is integrated into a Baker SterilGARD(R) lll hood making it particularly safe for the operator with the sorter operations/adjustments being affected remotely by computer. Nozzle exchange is simple and necessary alignments automated and computer controlled. Additionally, with the sorter head integrated into the Baker BSC, the design is particularly compact. When installed, the new system will provide a BSL-2 sorting capability not currently available in North Baltimore and on the Hopkins' Homewood campus. Currently, users must wait for weeks to gain access to sorters available at the Hopkins' East Baltimore or Bay View campuses located inconveniently five and ten miles away respectively. It will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance; it will be made freely available to all interested users Hopkins-wide through workshops & hands-on training; and it will be incorporated into the Center for Biotechnology Education's Masters in Biotechnology curriculum; and the IIC's annual undergraduate/graduate course offerings. (://www.jhu.edu/iic/academic.htm).