Acquisition of a Next Generation Orbitrap MS

描述(由申请人提供)：在本申请中，我们正在申请购买ThermoScience下一代Q-LTQ轨道探测器的资金。我们之所以需要这台仪器，是因为它的功能非常适合自下而上、中间向下和自上而下的蛋白质表征、有限样本的高灵敏度定量蛋白质组学和翻译后修饰分析。所需的新一代混合Orbitrap比目前的LTQ Orbitrap Elite有几个关键改进，使其能够实现更好的性能：1)质量选择四极杆可用于选择用于Orbitrap或离子陷阱分析的离子，2)离子陷阱和Orbitrap质谱仪并行操作，可显著提高扫描速度，3)HCD单元，用于在单离子或多路模式下离子积累，4)在MSN分析的任何阶段均可完全灵活地进行CID、HCD和ETD解离，5)具有先进的瞬时处理的超高场Orbitrap，6)新颖的高性能前端ETD源和专用的内部质量校准。这些改进加上开销的降低，在不牺牲灵敏度或动态范围的情况下，实现了比其他设计更高的高分辨率数据采集速率。所请求的下一代Orbitrap的更快的MS1和MS2组合扫描速率对于高效超高压LC分离的LC-MS/MS至关重要，从而在不损失数据质量的情况下减少蛋白质组实验时间。这一要求的下一代Q-LTQ Orbitrap将对支持几个主要用户的需求至关重要，这些用户在样本有限且灵敏度和吞吐量是关键要求的人体组织上执行大规模蛋白质组学。

DESCRIPTION (provided by applicant): In this application we are requesting funds for the purchase of a ThermoScientific a next-generation Q-LTQ Orbitrap. We are requesting this instrument because its capabilities are well suited for bottom-up, middle-down and top-down protein characterization, high-sensitivity quantitative proteomics of limited samples and post- translational modification analysis. The requested next-generation hybrid Orbitrap has several key improvements over the current LTQ Orbitrap Elite that allow it to achieve better performance: 1) Mass selecting quadrupole available to select ions for Orbitrap or ion trap analysis, 2) Parallel operation of both ion trap and Orbitrap mass analyzers allowing for significantly increased scan rate, 3) HCD cell for ion accumulation in single ion or multiplexed modes, 4) Full flexibility of CID, HCD and ETD dissociations at any stage of MSn analysis, 5) Ultrahigh field Orbitrap with advanced transient processing, 6) Novel, high performance front-end ETD source and dedicated internal mass calibration. These improvements along with reduced overhead result in a high-resolution data acquisition rate greater than other designs without sacrificing sensitivity or dynamic range. The faster combined MS1 and MS2 scan rates of the requested next-gen Orbitrap are essential for LC- MS/MS with high efficiency ultra-high pressure LC separations, resulting in reduced proteomic experiment time without loss of data quality. This requested next-generation Q-LTQ Orbitrap will be critical to support the needs of several Major Users that performing large-scale proteomics on human tissue where samples are limited and sensitivity and throughput are key requirements.