Microscopy and Imaging Core (Core 2)

夺去 显微镜和成像核心将被安置在南卡罗来纳州医学院现有的仪器资源设施（IRF），这是一个核心设施，为南卡罗来纳州的生物医学研究社区提供广泛的生物技术和技术专业知识。核心将为目标研究者提供所需的技术专长和设备，以完成他们提出的与膳食补充剂如何影响炎症过程有关的项目。监测炎症反应中细胞、组织和器官的结构变化，以及各种膳食补充剂如何改变这些变化，对于了解炎症是如何调节的至关重要。该核心将提供一系列完整的成像技术，用于监测从整个动物到超微结构水平的这些变化。在整个动物水平上，超声和荧光技术将可用于确定炎症和应用饮食如何影响实验动物。在组织学/光学显微镜和免疫组织化学水平上，有一整套设备可用于组织的制备和成像。通过旋转圆盘共聚焦显微镜在培养物中监测分离的细胞行为，并通过多标记共聚焦成像监测炎症过程中表达的分子的定位和共定位。最后，细胞超微结构的变化将通过电子显微镜成像，以确定亚微米级分辨率的变化。此外，还提供了一系列图像分析硬件和软件，用于量化细胞、组织和器官炎症期间发生的结构变化，以及这些变化如何受到各种膳食补充剂的影响。IRF提供所有设备和技术专长，以完成目标研究者提议的实验。将有三名教员和四名技术人员隶属于IRF可协助实验设计，设备操作和成像仪器培训。除了个别互动外，还经常通过综合资源框架主办教学课程、研讨会和讲习班。这些将提供给目标研究者实验室的成员。

Abtract The Microscopy and Imaging Core will be housed in the existing Instrumentation Resource Facility (IRF) at the University of South Carolina School of Medicine which is a core facility providing a wide range of biotechnology and technical expertise to the biomedical research community in the state of South Carolina. The core will provide the required technical expertise and equipment to the Target Investigators for completion of their proposed projects related to how dietary supplements affect the process of inflammation. Monitoring of structural changes in cells, tissues and organs that occur in response to inflammation, and how various dietary supplements alter these changes, is essential in understanding how inflammation can be regulated. The core will provide access to a complete range of imaging technology for monitoring these changes from the whole animal to ultrastructural level. At the whole animal level, ultrasound and fluorescence techniques will be available to determine how inflammation and applied diets affect the experimental animals. At the histology/light microscopy and immunohistochemistry levels, a full range of equipment is available for preparation and imaging of tissues. Isolated cell behavior will be monitored in culture by a spinning disk confocal microscope and localization and co-localization of molecules expressed during the process of inflammation will be monitored by multi-labeling confocal imaging. Finally, ultrastructural changes in cells will be imaged by electron microscopy to determine changes at the submicron level of resolution. A full range of image analysis hardware and software is also available to quantify the structural changes which occur during inflammation in the cells, tissues and organs and how these changes are affected by the various dietary supplements. All equipment and technical expertise is available in the IRF for completion of the experiments proposed by the Target Investigators. There will be three faculty members and four technicians affiliated with the IRF available for assistance with experimental design, operation of equipment, and training on imaging instrumentation. In addition to individual interactions, didactic courses, seminars and workshops are often sponsored through the IRF. These will be made available to members of the Target Investigator laboratories.