Microscopy and Imaging Core (Core 2)

减去 显微镜和成像核心将设在南卡罗来纳大学医学院现有的仪器资源设施(IRF)中，这是一个核心设施，为南卡罗来纳州的生物医学研究社区提供广泛的生物技术和技术专业知识。该核心将向目标调查人员提供所需的技术专长和设备，以完成他们关于膳食补充剂如何影响炎症过程的拟议项目。监测细胞、组织和器官因炎症而发生的结构变化，以及各种膳食补充剂如何改变这些变化，对于了解如何调节炎症是至关重要的。该核心将提供一整套成像技术，以监测从整个动物到超微结构的这些变化。在整个动物水平上，超声波和荧光技术将可用于确定炎症和应用的饮食对实验动物的影响。在组织学/光学显微镜和免疫组织化学层面，有一整套设备可用于组织的准备和成像。分离的细胞在培养中的行为将通过旋转圆盘共聚焦显微镜进行监测，在炎症过程中表达的分子的定位和共定位将通过多标记共聚焦成像进行监测。最后，细胞的超微结构变化将通过电子显微镜成像，以确定亚微米分辨率的变化。全套图像分析硬件和软件也可用于量化细胞、组织和器官在炎症期间发生的结构变化，以及这些变化如何受到各种膳食补充剂的影响。为完成目标调查人员提议的实验，IRF提供了所有设备和技术专长。将有三名教员和四名附属于IRF的技术人员，在实验设计、设备操作和成像仪器培训方面提供帮助。除了个人互动，授课课程、研讨会和讲习班通常是通过国际扶轮基金会赞助的。这些资料将提供给目标调查员实验室的成员。

Abtract The Microscopy and Imaging Core will be housed in the existing Instrumentation Resource Facility (IRF) at the University of South Carolina School of Medicine which is a core facility providing a wide range of biotechnology and technical expertise to the biomedical research community in the state of South Carolina. The core will provide the required technical expertise and equipment to the Target Investigators for completion of their proposed projects related to how dietary supplements affect the process of inflammation. Monitoring of structural changes in cells, tissues and organs that occur in response to inflammation, and how various dietary supplements alter these changes, is essential in understanding how inflammation can be regulated. The core will provide access to a complete range of imaging technology for monitoring these changes from the whole animal to ultrastructural level. At the whole animal level, ultrasound and fluorescence techniques will be available to determine how inflammation and applied diets affect the experimental animals. At the histology/light microscopy and immunohistochemistry levels, a full range of equipment is available for preparation and imaging of tissues. Isolated cell behavior will be monitored in culture by a spinning disk confocal microscope and localization and co-localization of molecules expressed during the process of inflammation will be monitored by multi-labeling confocal imaging. Finally, ultrastructural changes in cells will be imaged by electron microscopy to determine changes at the submicron level of resolution. A full range of image analysis hardware and software is also available to quantify the structural changes which occur during inflammation in the cells, tissues and organs and how these changes are affected by the various dietary supplements. All equipment and technical expertise is available in the IRF for completion of the experiments proposed by the Target Investigators. There will be three faculty members and four technicians affiliated with the IRF available for assistance with experimental design, operation of equipment, and training on imaging instrumentation. In addition to individual interactions, didactic courses, seminars and workshops are often sponsored through the IRF. These will be made available to members of the Target Investigator laboratories.