Confocal/Multiphoton Microscope Upgrade

 描述(由申请人提供)：细胞和分子成像(CMI)设备是细胞死亡、损伤和再生中心(CCDIR)和NCI指定的霍林斯癌症中心(HCC)的共同组成部分，其目的是为CCDIR/HCC研究人员和南卡罗来纳医科大学(MUSC)的其他研究人员提供激光扫描共焦/多光子显微镜和相关高端光学成像技术的设备和专业知识。我们的研究人员使用联合共焦/多光子显微镜的唯一途径是一台8岁以上的蔡司510NLO元显微镜，该显微镜缺乏高性能共焦/多光子所必需的重要功能 以及活体(活体)成像，包括光谱检测、长工作距离浸水光学和最高灵敏度的去扫描和非去扫描检测。因此，该应用程序的总体目标是将蔡司510 NLO显微镜升级为配置最佳且功能齐全的共焦/多光子显微镜系统，以供CCDIR、HCC和MUSC研究人员使用。我们建议将该系统升级为Zeiss 780共焦/多光子倒置激光扫描显微镜，配备最高灵敏度的3通道非去扫描GaAsP探测器、基于GaAsP的34通道光谱阵列探测器、6条可见激光共焦成像线、高性能长工作距离活体浸水光学系统和Zen软件。通过使用旧显微镜的显微镜物镜、多光子激光、隔振台和环境室，将实现成本节约，其剩余部件将被以旧换新。升级后的显微镜系统将置于多用户环境中，为支持个别用户项目提供技术支持、用户培训和应用专业知识。

 DESCRIPTION (provided by applicant): The Cell and Molecular Imaging (CMI) Facility is a shared component of the Center for Cell Death, Injury and Regeneration (CCDIR) and the NCI-designated Hollings Cancer Center (HCC) whose purpose is to provide CCDIR/HCC investigators and other researchers at the Medical University of South Carolina (MUSC) with equipment and expertise for laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques. The only access of our investigators to combined confocal/mulitphoton microscopy is an 8+ year old Zeiss 510 NLO META microscope that is deficient in important functionalities that are essential for high performance confocal/multiphoton and intravital (in vivo) imaging, including spectral detection, long working distance water immersion optics and maximally sensitive descanned and nondescanned detection. Accordingly, the overall goal of this application is to upgrade the Zeiss 510 NLO microscope to an optimally configured and fully functional confocal/multiphoton microscope system to be made available to CCDIR, HCC and MUSC investigators. We propose to upgrade this system to a Zeiss 780 confocal/multiphoton inverted laser scanning microscope with maximally sensitive 3-channel non-descanned GaAsP detectors, a GaAsP-based 34-channel spectral array detector, 6 visible laser lines for confocal imaging, high performance long working distance intravital water-immersion optics and Zen software. Cost savings will be achieved by using microscope objectives, multiphoton laser, vibration isolation table and environmental chamber from the old microscope, whose remaining components will be traded in. The upgraded microscope system will be placed in a multiuser environment that provides technical support, user training and applications expertise in support of individual user projects.