MAINTENANCE OF A CLOSED CPRC SPF COLONY

 描述(由申请人提供)：本申请的主要目标是通过支持运营、管理、兽医护理、病毒学和MHC分型，以及通过改进全面的遗传管理计划，维持加勒比灵长类动物研究中心(CPRC)的无特定病原体(SPF)计划。CPRC动物的一个独特特点是，这些动物自然没有SIV和SRV-D，这两种病毒是要测试的四种病毒中的两种。毫无疑问，这一特点有助于建立一个成功的计划，而血清转换的次数非常有限。然而，为了安全和SPF计划的要求，CPRC继续系统地检测所有四种病毒(B病毒、STLV、SIV和SRV-D)。该计划为纯印度血统的恒河猴提供明确的遗传和病毒学背景。群体遗传分析表明，随着时间的推移，SPF群体保持了遗传上的同质性，具有足够的杂合度和来自非SPF创始人的最小分层。使用本申请中描述的遗传管理策略，将在SPF群体中保持遗传变异。遗传管理方法将允许丰富所需的MHC标记，以满足生物医学研究的需求，同时保持遗传多样性。为了确保CPRC-SPF群体的长期生存能力，该中心的基因管理方法将与NIH的国家NHP遗传学和基因组工作组的指导方针保持一致。在C，P，R，C，S，P，F，A，N，A，L，S中，印度血统的估计也是美国几个国家灵长类研究中心计算出的最高的之一。在这项申请中，要求提供资金以维持SPF计划和实现拟议的目标。在NIH的持续支持、机构捐款和项目收入的资金支持下，CPRC的SPF项目将仍然是一个重要的国家研究资源，并将能够为NIH赞助的研究项目，特别是艾滋病毒/艾滋病研究提供具有明确遗传和病毒学背景的健康SPF印度血源恒河猴。

 DESCRIPTION (provided by applicant): The major goal of this application is to maintain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, management, veterinary care, virological and MHC typing, and by the improvement of the comprehensive genetic management plan. A unique feature of the CPRC animals that had facilitated the successful establishment and improved sustainability of the SPF colony is that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for(4). Undoubtedly, this trait has contributed to the establishment of a successful program with very limited number of sero-conversions. Nevertheless, for safety and as required for the SPF Program, The CPRC continues systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minima stratification from its non- SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. To ensure the long-term viability of the CPRC-SPF colony, the Center's approach for genetic management will be concordant with the NIH's National NHP Genetics and Genomics Working Group's guidelines. The estimate of Indian ancestry among the C P R C - S P F a n i m a l s is also among the highest that have been calculated across several National Primate Research Centers (NPRCs) in the US. Funds are requested in this application to maintain the SPF program and to meet the proposed aims. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF Indian- origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs, specially HIV/AIDS studies.