Mount Sinai HHEAR Network Targeted Lab Hub

项目总结 目标分析资源是一个多学科、全方位服务暴露的生物标记物计划，旨在 将无机和有机环境措施整合到现有的NIH研究中。在这份增刊中，我们现在 扩大服务范围，包括癌症研究。我们每周举行一次执行指导委员会会议 完整的实验室中心团队，讨论工作、对工作流程进行故障排除并确定资源和活动的优先顺序，以便 为HHEAR客户提供最先进的服务。我们的团队有相当多的专业知识来匹配 靶向暴露生物标记物对研究设计、生物标本类型的潜在毒理学 收集，并对健康表型进行测量。我们的实验室可行性评估有助于形成 客户对更高影响研究的意见书。我们还建议客户防止使用贵重的 被认为可能低于检测限值的化验样品(例如，测定有机磷 郊区人口中的农药代谢物)基于我们多年的研究经验和 了解暴露场景。我们与行政核心合作协调服务，包括 样品管理、接收、标签和跟踪。我们担任协调中心的专家顾问 中心、PI/客户和其他HHEAR资源。我们与数据中心协作管理 结果报告，确保数据格式正确，具有足够的元数据，并参与 在暴露/表型关系的分析中。我们使用自动化的机器人处理样品 制备方法、高通量分析方法和实验室间最新QA/QC 包括实时层析监控和通过状态的数据库管理的程序 先进的实验室信息管理系统(LIMS)软件。我们还自动进行数据处理，以 减少人为错误。我们的方法确保了适当的样本跟踪和数据报告，并避免了 从样本到结果。有了可支配的大量机构资源，目标资源翻了一番 在过去的3年中，质谱仪的数量增加到14台，确保了我们为 HHEAR网络的需求。我们的团队也进行了广泛的推广，是唯一的CHEAR实验室中心 组织全国暴露生物标志物研讨会(2018年11月2日至3日在西奈山举行，主题为 暴露组学)，如《管理核心》中所述。QA/QC协议和标准操作程序 所有目标化合物都已确定，以保持我们的化学分析的严谨性。我们开发了 元素和有机化学品小组，总共评估200多种化学物质。我们参加了所有 CHEAR授权金属和有机化学品的QA/QC和PT计划以验证我们的精确度和 准确性，并将为HHEAR这样做。总而言之，我们高效地处理和分析样品。 和准确性，提供专家科学咨询，并将我们在靶向暴露分析方面的工作与 其他集线器组件始终保持高度的生产力和成本效益。

PROJECT SUMMARY The Targeted Analysis Resource is a multi-disciplinary, full service exposure biomarker program designed to integrate inorganic and organic environmental measures into existing NIH studies. In this supplement we now extend services to include Cancer studies. We hold weekly Executive Steering Committee meetings of the full Lab Hub team to discuss jobs, troubleshoot workflows, and prioritize resources and activities in order to provide state of the art service to HHEAR clients. Our team has considerable expertise in matching the underlying toxicology of targeted exposure biomarkers to the study design, to the biological specimen types collected, and to the health phenotypes measured. Our lab feasibility assessments help shape the science of a client's submission towards higher impact studies. We also counsel clients on preventing the use of precious samples on assays that are deemed likely to be below detection limits (e.g. measuring organophosphate pesticide metabolites in suburban populations) based on our many years of research experience and understanding of exposure scenarios. We work with the Administrative Core to coordinate services, including sample management, receiving, labeling and tracking. We serve as expert consultants with the Coordinating Center, PI/Clients and other HHEAR Resources. We collaborate with the Data Center in the management of results reporting, ensuring that data are properly formatted and have sufficient metadata, as well as participate in the analysis of exposure/phenotype relationships. We use automated robotic sample handling and preparation methods, high throughput analytical procedures and state of the art inter-laboratory QA/QC procedures which include real time chromatography monitoring and database management through state-of- the-art laboratory information management system (LIMS) software. We also automated data processing to reduce human error. Our methods ensure proper sample tracking and data reporting and avoid mismatches of samples to results. With substantial institutional resources at its disposal, the Targeted Resource has doubled the number of mass spectrometry instruments to 14 in the last 3 years, ensuring our capacity to serve the needs of the HHEAR Network. Our team also conducts extensive outreach and was the only CHEAR lab hub to organize a National Exposure Biomarker Symposium (held Nov 2-3, 2018 at Mount Sinai and themed on Exposomics) as described in the Administrative Core. QA/QC protocols and standard operating procedures for all target compounds have been established to maintain the rigor of our chemical analyses. We developed panels for elements and organic chemicals that in sum assess more than 200 chemicals. We participated in all CHEAR mandated QA/QC and PT programs for metals and organic chemicals to verify our precision and accuracy and will do so for HHEAR. In sum, we process and analyze samples with a high degree of efficiency and accuracy, provide expert scientific consultation and integrate our work on targeted exposure assays with the other Hub components all the while maintaining a high degree of productivity and cost effectiveness.