EXAMINATION OF ALLOSTERIC SITE OF SEROTONIN TRANSPORTER USING TRANSGENIC MICE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究采用了一种定向转基因敲入小鼠，在这种小鼠中，5-羟色胺转运体中先前描述的突变显著改变了特定抗抑郁药的结合。我们正在研究这种转运蛋白突变与小鼠的分子和行为之间的关系。 在本报告所述期间，我们已经完成了转基因靶向载体的设计和生产，并已开始使用两种不同的转基因设施将构建物导入胚胎干细胞。我们目前正在筛选同源重组克隆的细胞系。我们还开始检查野生型小鼠对急性和慢性西酞普兰剂量的行为反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study employs a targeted transgenic knock-in mouse in which a previously characterized mutation within the serotonin transporter significantly alters the binding for specific antidepressants. We are examining the molecular and behavioral correlates of mice with mutations in this transporter. During the reporting period, we have completed the design and production of the transgenic targeting vector and have begun working with two different transgenic facilities to transfect the constructs into embryonic stem cells. We are currently screening cell lines for homologous recombinant clones. We have also begun examining behavioral responses to acute and chronic citalopram dosing in wild-type mice.