EFFECT OF SYSTEMIC HYPOXIA ON MSNA & ITS RELATIONSHIP TO INTERSTITIAL ADENINE NU

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在全身缺氧时，交感神经活动增加，同时骨骼肌间质腺苷和去甲肾上腺素增加。目前尚不清楚间质腺苷的增加是来自血管内皮细胞，还是来自骨骼肌纤维，还是来自细胞外ATP的转化。在这项研究中，我们建议通过同时测量肌肉内微透析探头在三种明显不同的同感兴奋干预下的去甲肾上腺素和腺嘌呤核苷酸(ATP、ADP和AMP)，我们将获得区分独特的血管对低氧反应的必要信息。将使用的三种不同的15分钟刺激包括：1)全身缺氧；2)静脉输注硝普钠；3)下体负压。将对16名志愿者进行研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During systemic hypoxia, sympathetic nerve activity is increased accompanied by increases in skeletal muscle interstitial adenosine and norepinephrine. It is not known whether the increases in interstitial adenosine come from vascular endothelium, from skeletal muscle fiber or from extra cellular conversion of ATP. In this study, we propose that through simultaneous measurement of norepinephrine and adenine nucleotides (ATP, ADP, and AMP) from intramuscular microdialysis probes during three distinctly different sypatho excitatory interventions we will obtain the necessary to differentiate unique vascular responses to hypoxia. The three different 15 minute stimuli that will be used include: 1) systemic hypoxia; 2) intravenous infusion of sodium nitroprusside; and 3) lower body negative pressure. 16 volunteers will be studied.