National Biomedical NMR Resource at Madison

描述（由申请人提供）：位于麦迪逊的国家磁共振设施（NMRFAM）是生物分子核磁共振领域前沿的一个站点，通过资源技术开发计划在以下重要领域得到扩展：(1)快速数据收集和自动数据分析；(2)较大蛋白质和复合物的技术；(3)含金属（顺磁性）蛋白质的研究；(4)大分子动力学。(5) RNA分子及其与金属离子和蛋白质配合物的结构功能研究。通过追求其创新的研究技术开发活动，并将其应用于生物重要性的合作项目，NMRFAM努力成为展示生物分子核磁共振领域未来能力的典范。NMRFAM为生物医学NMR调查提供从头到尾的支持。根据需要，NMRFAM将为以下一个或多个步骤提供支持：(1)策略评估，(2)样品制备，(3)可行性研究，(4)数据收集，(5)数据分析和结构确定。我们的目标是通过为研究人员提供符合其特定需求的资源，促进对新知识的有效追求。NMRFAM为年轻的研究人员和经验丰富的光谱学家提供了最先进的仪器，支持多种模式的数据收集。通过NMRFAM的研究活动开发的协议、脉冲序列和软件工具可供一般科学界使用。其目的是发展和传播先进的实验设计和数据分析方法，涵盖生物分子核磁共振研究的所有步骤，从克隆到数据沉积。为了扩大其科学活动的范围，NMRFAM将接待杰出的访问科学家，在与其研究技术开发项目相关的领域工作。作为培训其用户基础和传播其新技术的手段，NMRFAM将每半年举办一次讲习班和小组培训会议。生物分子核磁共振波谱是提供溶液中生物分子最详细信息的单一方法，它们通常在其中起作用。核磁共振采用越来越先进的技术来确定结构，并发现生物系统如何工作和对药物的反应。我们的目标是开发方法，使这些研究更快，成本更低，并适用于人类疾病中重要的更大类蛋白质和核酸。

DESCRIPTION (provided by applicant): The National Magnetic Resonance Facility at Madison (NMRFAM) is a site where the frontiers of the biomolecular NMR field are being expanded through resource technology development programs in the important areas of (1) fast data collection and automated data analysis, (2) technology for larger proteins and complexes, (3) investigations of metal-containing (paramagnetic) proteins, (4) dynamics of macromolecules, and (5) structure-function investigation of RNA molecules and their complexes with metal ions and proteins. By pursuing its innovative research technology development activities, and by applying them to collaborative projects of biological importance, NMRFAM strives to be a model for demonstrating the future capabilities of the biomolecular NMR field. NMRFAM offers start-to-finish support for biomedical NMR investigations. As needed, NMRFAM will lend support to one or more of the following steps: (1) strategy evaluation, (2) sample preparaton, (3) feasibility studies, (4) data collection, and (5) data analysis and structure determination. Our aim is to facilitate the efficient pursuit of new knowledge, by providing researchers with resources matched to their particular needs. NMRFAM provides young investigators and experienced spectroscopists access to state-of-the-art instrumentation, with support for multiple modes of data collection. Protocols, pulse sequences, and software tools, developed through NMRFAM's research activities, are made available to the general scientific community. The aim is to develop and disseminate advanced approaches to experimental design and data analysis that cover all steps in a biomolecular NMR investigation, from cloning through data deposition. With the goal of broadening the scope of its scientific activities, NMRFAM will host distinguished visiting scientists, working in areas related to its research technology development projects. As a means for training its user base and for disseminating its novel technology, NMRFAM will conduct semi-annual workshops and group training sessions. Biomolecular NMR spectroscopy is the single approach that offers the most detailed information about biomolecules in solution, the milieu in which they normally function. NMR employs increasingly advanced technology to determine structures and to discover how biological systems work and respond to drugs. Our goal is to develop methods for making these investigations faster and less costly, as well as applicable to larger classes of proteins and nucleic acids of importance in human disease.