MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 正链RNA病毒的RNA复制普遍与膜和膜重排如单或双膜囊泡有关。 然而，在大多数情况下，RNA合成的网站（S）相对于这些重排的膜和膜，囊泡内部，内腔室和细胞质之间的拓扑关系是不明确的。 最近的结果表明，至少在某些情况下，病毒RNA复制发生在这样的囊泡内。 然而，这些发现留下了许多未解决的问题，在许多方面，由于固有的局限性，在二维分析使用。 野田村病毒家族中研究最多的成员，鸡群屋病毒（FHV），在受感染的果蝇细胞的线粒体外膜上复制，在膜间隙中形成膜结合小球，类似于许多正链RNA病毒观察到的多囊泡结构。我们采用电子显微镜断层扫描（EMT），以解决形态的FHV小球膜。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA replication by positive-strand RNA viruses is universally associated with membranes and membrane rearrangements such as single or double membrane vesicles. However, in most cases the site(s) of RNA synthesis relative to these rearranged membranes and the topological relationships between membranes, vesicle interiors, lumenal compartments and the cytoplasm are poorly defined. Recent results suggest that, in at least some cases, viral RNA replication occurs inside such vesicles. However, these findings leave many unresolved questions, in many ways due to limitations inherent in the two-dimensional analyses used. The best-studied member of the nodavirus family, flock house virus (FHV), replicates at the outer mitochondrial membranes of infected Drosophila cells where membrane-bound spherules form in the intermembrane space, similar to multi-vesicular structures observed for many positive-strand RNA viruses. We employ electron microscope tomography (EMT) to resolve the morphology of the FHV spherule membranes.