MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 电子显微镜断层扫描的使用揭示了膜结构和拓扑结构的野田村病毒RNA复制区室。正链RNA病毒的一个普遍特征是宿主细胞内膜参与RNA复制。已经观察到许多正链RNA病毒包括甲病毒、小核糖核酸病毒、黄病毒等的与病毒RNA复制相关的细胞内膜重排。在野田病毒群舍病毒（FHV）中，病毒RNA合成发生在线粒体膜上，与40个核苷酸的形成相关。  线粒体内膜和外膜之间的60 nm小泡或小球。对于甲病毒超家族中的雀麦花叶病毒，已经发现RNA复制发生在内质网膜上惊人相似的小球内。为了更好地定义这种病毒RNA复制结构的结构和拓扑结构，我们研究了FHV感染的果蝇细胞中形成的小球，通过电子显微镜断层扫描，这使得细胞结构的三维可视化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of electron microscope tomography revealed the membrane structure and topology of nodavirus RNA replication compartments. A universal feature of positive-strand RNA viruses is the involvement of host intracellular membranes in RNA replication. Intracellular membrane rearrangements associated with viral RNA replication have been observed for many positive-strand RNA viruses including alphaviruses, picornaviruses, flaviviruses, and others. In the nodavirus flock house virus (FHV), viral RNA synthesis occurs on mitochondrial membranes in association with formation of 40  60 nm vesicles or spherules between the inner and outer mitochondrial membranes. For brome mosaic virus in the alphavirus superfamily, RNA replication has been found to occur inside strikingly similar spherules on endoplasmic reticulum membranes. To better define the structure and topology of such viral RNA replication structures, we studied the spherules formed in FHV-infected Drosophila cells by electron microscope tomography, which permits the visualization of cellular structures in three dimensions.