Pathology

病理学核心使希望之城癌症中心的研究人员能够直接研究DNA，RNA和 蛋白质在人类肿瘤中的应用，以验证临床前研究产生的特定假设。它还提供 分析肿瘤组织中的特定蛋白质表达，使科学家能够辨别分布模式 这些蛋白质在特定细胞类型中的表达。病理学核心主要在专业领域提供服务 解剖病理学系的成员它们分为以下几个方面：（1）性能 并协助组织的常规组织学处理，包括石蜡包埋、切片和 人组织、动物组织和细胞系制备物的苏木精和伊红（H&E）染色，以及 作为专门的组织学服务，如制备多肿瘤块或组织阵列， 研究人员的规范;（2）免疫组织化学检测的性能和解释 正常和疾病状态下的细胞产物，包括新抗体的评估和技术开发 以及在同一组织切片中检测多种抗原的技术;（3）性能和 协助从石蜡或冷冻切片中进行激光捕获显微切割，以富集特定的 细胞类型，包括恶性细胞的分离，用于分析蛋白质、RNA和/或DNA含量;（4） 在制备福尔马林固定的组织用于常规PCR（包括RT-PCR）方面的性能和帮助;以及 (5)为癌症中心的研究人员提供咨询服务。在疾病的病理基础方面的专业知识， 疾病的组织学表现，以及人体组织用于研究疾病状态的用途可用于 准备赠款和论文以及设计实验。在前12个月报告期间 在此期间，病理核心为来自所有五个研究项目的33名癌症中心成员提供了服务 和一个不结盟成员国。在同一报告期间，同行评审的使用量占使用量的69 期核心资源的拟议年度预算为406，308美元，其中28%来自机构，32%来自用户 40%（160，982美元）要求CCSG。

The Pathology Core enables City of Hope Cancer Center investigators to directly study the DNA, RNA and protein in human tumors to validate specific hypotheses generated from pre-clinical studies. It also provides analyses of specific protein expression in tumor tissues to enable scientists to discern the distribution patterns of these proteins in specific cell types. The Pathology Core provides services primarily in the areas of expertise of the members of the Department of Anatomic Pathology. They fall into the following areas: (1) performance and assistance in the routine histological processing of tissues, including paraffin embedding, sectioning, and hematoxylin and eosin (H&E) staining of human tissue, animal tissue, and preparations from cell lines, as well as specialized histological services such as preparation of multi-tumor blocks or tissue arrays to the specifications o1 the researchers; (2) performance and interpretation of immunohistochemistry for the detection of cell products in normal and disease states, including evaluation and technical development of new antibodies as well as the technology to detect multiple antigens in the same histological section; (3) performance and assistance in Laser Capture Microdissection from paraffin or frozen sections to obtain enrichment of specific cell types, including isolation of malignant cells, for the analysis of proteins, RNA and/or DNA content; (4) performance and assistance in preparing formalin-fixed tissues for conventional PCR including RT-PCR; and (5) consultation services to Cancer Center investigators. Expertise in the pathological basis of disease, the histological manifestations of disease, and the use of human tissues for studying disease states is available for the preparation of grants and papers and in the design of experiments. During the prior 12 month reporting period, the Pathology Core provided service to 33 Cancer Center members from all five Research Programs and one non-aligned member. Peer-reviewed usage accounted for 69% of usage during this same reporting period. The proposed annual budget for the core is $406,308, with 28% from the institution, 32% from user fees, and 40% ($160,982) requested from the CCSG.