WNPRC ASSAY SERVICES UNIT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为核心、校园和非东道主调查人员提供复杂和专业的分析资源和专业知识。 进展和关切 从2007年5月1日至2008年1月31日，Assay Services已收到175,978.25美元作为我们服务的按存储容量使用计费收入，6,045美元来自计费基本赠款帐户，7,434美元用于向我们自己收取验证和质量控制费用。在这段时间里，我们进行了46,630次测定。在此期间，化验服务公司的新举措是：1)为其他实验室的蛋白质激素放射免疫分析提供碘，作为服务费；2)获得专业知识，为我们的EIA启动类固醇和蛋白质结合；3)成为威斯康星大学临床翻译科学奖的核心实验室，进行翻译研究；4)继续使用LC/MS进行多种类固醇分析。 资源访问权限的分配 化验服务拥有国际和国内的客户基础。在过去的一年里，我们为来自许多不同州和几个国家的调查人员测试了样本。我们主要为基于NIH的资助提供服务，使用非人类灵长类样本进行荷尔蒙测定，最近还提供翻译研究。 化验服务公司有一个便于科学界评估的网站： Http://ink.primate.wisc.edu/~assay/assay.php 传播 我们有很多客户通过我们的网站与我们联系。此外，我们还参加美国灵长类动物学家协会、行为神经内分泌学学会和内分泌学会等会议，让非人类灵长类动物群体了解我们的服务。我们也接受客户的推荐，由以前的客户。 培训 化验服务继续培训研究生、技术支持人员、博士后和科学家以自助方式运行自己的样本。卡尔加里大学的萨拉·卡内基去年夏天访问了化验服务部，分析了在对绵羊进行了18个月的实地研究期间收集的粪便样本中的生殖激素。杜兰大学的Valerie Schoff也在夏天访问了该实验室，分析了Cebus capucinus样本。威斯康星大学人类学的Sarie Van Belle对来自Alouatta pgra的粪便样本进行了培训和化验，以检测孕酮、雌二醇和皮质醇。莉莎·莫斯科维奇训练并分析了尿液和粪便样本中的催产素、加压素、催乳素和睾酮。伊丽莎白·贝克尔为加利福尼亚小鼠训练并分析了皮质酮、睾酮和黄体酮的血清样本。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To provide core, campus and non-host investigators with sophisticated and specialized assay resources and expertise. Progress and Concerns From May 1, 2007 through January 31, 2008, Assay Services has received $175,978.25 as income from our charge backs for our service, $6,045 from charging base grant accounts and $7,434 for charging ourselves for validations and quality controls. We ran 46,630 determinations during this time. Assay Services' new initiatives during this time have been 1) iodinating for other labs for their RIA of protein hormones as a fee for service, 2) obtaining the expertise to initiate steroid and protein conjugation for our EIAs, 3) beoming the core lab for the Clinical Translational Science Award at the University of Wisconsin for translational studies, 4) continued use of multiple steroid analyses by LC/MS. Allocation of Resource Access Assay Services has an international and national client base. During the past year we ran samples for investigators from many different states and several countries. We primarily provide services for NIH based grants using nonhuman primate samples for hormonal determinations and more recently for translational studies. Assay Services has a website that is easily assessable to the scientific community: http://ink.primate.wisc.edu/~assay/assay.php Dissemination We have many clients who contact us via our website. Additionally, we attend meetings such as the American Society of Primatologists, Society of Behavioral Neuroendocrinology, and the Endocrine Society to make the nonhuman primate community aware of our services. We also receive clients from recommendations by previous clients. Training Assay Services continues to train graduate students, technical support, post doc and scientists to run their own samples as self-service. Sarah Carnegie, University of Calgary, visited the Assay Services Unit this last summer to to analyze reproductive hormones from fecals samples collected during an 18 month field study of Cebus capucinus. Valerie Schoff, Tulane University, also visited the lab during the summer to analyze Cebus capucinus samples. Sarie Van Belle, University of Wisconsin  Anthropology, trained and ran fecal samples for progesterone, estradiol and cortisol from Alouatta pigra. Liza Moscovice trained and analyzed urine and fecal samples for oxytocin, vasopressin, prolactin and testosterone in Papio ursinus. Elizabeth Becker trained and analyzed serum samples for corticosterone, testosterone and progesterone for Californica mus.