ORIENTATION OF FMO PROTEIN IN PHOTOSYNTHETIC BACTERIA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在光合作用生物体中观察到的高激发能量转移效率依赖于单个蛋白质复合体中最佳的色素-蛋白质结合几何结构，也取决于光系统的整体结构。在绿色硫磺细菌中，膜上附着的Fenna-Matthews-Olson(FMO)天线蛋白作为一条“电线”将大的外周绿体天线复合体与嵌入细胞膜的反应中心(RC)连接起来。由叶绿体收集的能量通过FMO被输送到RC。为了了解单个孤立粒子的结构和功能之间的关系，已经进行了大量的工作。然而，FMO蛋白如何与体内的膜和绿体相互作用以维持特定的结构以确保高效的能量传递途径的问题尚未得到回答。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The high excitation energy transfer efficiency observed in photosynthetic organisms relies on the optimal pigment-protein binding geometry in the individual protein complexes and also on the overall architecture of photosystems. In green sulfur bacteria, the membrane-attached Fenna-Matthews-Olson (FMO) antenna protein functions as a "wire" to connect the large peripheral chlorosome antenna complex with the reaction center (RC), which is embedded in the cytoplasmic membrane. Energy collected by the chlorosome is funneled through the FMO to the RC. Significant effort has been expanded to understand the relationship between structure and function of the individual isolated particles. The question of how the FMO protein interacts with the membrane and the chlorosome in vivo to maintain a specific architecture to ensure the highly efficient energy transfer pathway, however, has not been answered.