High Throughout Facility

项目总结（见说明）： 高通量筛选设施（HTSF）是在过去五年中发展起来的，以满足高通量能力的需求，并成为多个转化研究领域的中心资源。HTSF提供筛选服务，包括开发检测试剂盒的仪器和专业知识，执行siRNA和小分子筛选，并支持后续和二次检测试剂盒的开发和执行。此外，HTSF资源可供临床研究者使用， 为临床试验和人口科学研究开发和实施临床相关分析。自动化和检测仪器可供所有福克斯蔡斯癌症中心（FGGG）的研究人员使用，为研究人员提供新技术。HTSF提供的仪器和资源包括：与96和384孔筛选平台兼容的自动化，人类基因组siRNA文库（Thermo-Fisher Dharmacon）和小分子文库（约50，000种化合物）。在HTSF作为开发机构期间，HTSF获得了资源和试剂，启动了筛选执行程序，并提供了分析服务和筛选支持。在戈伊2009年，HTSF提供了支持，19同行评审，资助研究人员，从所有五个中心的CCSG研究计划（第五部分）。 由于气候变化框架公约提供了强有力的机构支持，包括776，808美元的启动设备和1，454，952美元的业务费用（2005财政年度至今），这一用户基础得以发展。这使该设施能够以较低的费率向主要研究员收取费用，以提高使用率。设备、专业知识和补贴的结合，共同创造了一个筛选和翻译社区。 通过启动PI实验室之间以及设施之间的合作关系，同时在此过程中创造对HTSF支持的额外需求，HTSF是一个独特的资源，因为它结合了对不同研究人员群体的服务和多个设施之间的合作。这允许 HTSF通过充分利用该中心在高通量技术、传统实验室工作和临床方法方面的资源，成为转化科学的中心。

PROJECT SUMMARY (See instructions): The High Throughput Screening Facility (HTSF) has developed during the previous five years to meet a need identified for high throughput capabilities and a central resource for multiple areas of translational research. The HTSF provides screening services, including the instrumentation and expertise to develop assays, execute siRNA and small molecule screens, and support for follow-up and secondary assay development and execution. In addition, the HTSF resources are available to clinical investigators to develop and implement clinical correlate assays for clinical trials and population science studies. The automation and detection instrumentation is available to all Fox Chase Cancer Center (FGGG) researchers, providing new technologies to investigators. Instrumentation and resources available from the HTSF include: automation compatible with 96 and 384 well screening platforms, the human genome siRNA library (Thermo-Fisher Dharmacon), and small molecule libraries (approx 50,000 compounds). Duhng its period as a developing facility the HTSF has acquired resources and reagents, initiated procedures for screen execution and provided assay services and screening support. In GY 2009, the HTSF provided support to 19 peer-reviewed, funded investigators, from all five of the Center's CCSG Research Programs (Section V). This user base has developed due to the strong institutional support provided by FCCC, including $776,808 for start-up equipment, as well as $1,454,952 in operating costs (FY2005-present). This has enabled the Facility to charge back Principal Investigators, (Pis) at reduced rates to enhance usage. The combination of equipment, expertise and subsidization has converged to create a community of screeners and translational scientists by initiating collaborative relationships among PI laboratories as well as between facilities, while in the process creating additional demand for HTSF support. The HTSF is a unique resource, as it combines services to a diverse group of investigators and collaboration among multiple, facilities. This allows the HTSF to act as a hub for translational science by making the most of the Center's resources in high throughput technology, traditional benchwork and clinical approaches.