Center for Caenorhabditis Elegans Anatomy

线虫，秀丽隐杆线虫，已被确定为细胞的“高连通性”动物， 发展这种非常小，简单的动物已被广泛采用的实验研究，由于其出色的 遗传学和细胞生物学。该蠕虫的完整基因组已经公布，大部分成虫 从连续薄片的电子显微照片重建了解剖结构。然而，EM技术 没有被C广泛使用。elegans社区，大多数以前的EM数据不容易访问。中心 梭elegans Anatomy致力于在C.特别是1）研究细胞 正常发育期间和突变动物中的变化，以及2）进行EM-免疫细胞化学， 在超微结构水平上定位抗原或分子的组织化学研究。目前正在进行这类研究 与NIH和NSF资助的实验室密切合作，以实现其现有的实验目标。 此外，该中心正在收集有关线虫解剖结构的详细信息， 注释的TEM和光学显微照片。信息可在我们的WormAtlas和 蠕虫网站，通过ftp，在CD和DVD上，现在在C。秀丽隐杆线虫必要时， 该中心正在测试新的EM方法。并对野生型解剖进行新的EM调查 在关键的发展阶段，以补充地图集，并填补空白，在我们的一般知识。该中心还 提供TEM方法的培训，以便更多的C。elegans实验室可以进行他们自己的EM研究。 该R24申请首次获得资助，中心于1998年2月开放;我们要求五年的资助， 继续建设我们服务科学界的能力。 相关性（参见说明）： 许多疾病相关的基因序列在C.线虫基因组;遗传的透射电镜病理学 线虫中的突变通常有助于发现人类中等同基因产物的功能。 该实验室的研究揭示了与大脑发育有关的基本机制， 探索了涉及几种形式的细胞死亡的基因，包括凋亡、坏死和自噬。

The nematode, Caenorhabditis elegans, has been identified as a "high connectivity" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on extending the EM methods on C. elegans, particularly to 1) study cellular changes during normal development and in mutant animals, and 2) conduct EM-immunocytochemical and histochemical studies to localize antigens or molecules at the ultrastructurallevel. Such studies are being conducted in close collaboration with NIH- and NSF-funded laboratories, in fulfillment of their existing experimental aims. In addition, the Center is 3)publishingdetailed information regarding the anatomy of the nematode in the form of annotated TEM and light micrographs. Information is available on the Internet on our WormAtlas and Wormlmage websites, by ftp, on CDs and DVDs, and now in book form in the C. elegans Atlas. Where necessary, the Center is 4) testing new EM methods for C. elegans, and conducting new EM surveys of the wild type anatomy at key developmental stages to supplement the Atlas, and to fill gaps in our general knowledge. The Center also provides 5) training in TEM methods so that more C. elegans labs can conduct their own EM studies. This R24 application was first funded and the Center opened in Feb. 1998; we request five years of funding to continue building our capabilities to serve the scientificcommunity. RELEVANCE (See instructions): Many disease-related gene sequences are known in the C. elegans genome; the TEM pathology of genetic mutations in nematode is often helpful in discovering the function of equivalent gene products in man. Studies in this laboratory have uncovered basic mechanisms related to development of the brain, and explored genes involved in several forms of cell death, including apoptosis, necrosis and autophagy.