Genetic Biochemical Studies of Plant Steroid Signaling

R 01 GM 066258：植物类固醇信号的遗传生化研究。 主要研究者：王志勇 修改项目摘要/摘要部分 该项目的长期目标是了解分子网络， 激素、环境因素和营养物质共同控制植物的生长， 发展植物已经进化出强大的细胞信号系统来调节生长 以及根据内部状态和环境条件进行代谢。解剖 这些植物管理系统不仅对粮食安全和环境保护很重要， 可持续性，而且还有助于了解一般的细胞调节，因为许多 在动物和植物中是高度保守的。该研究计划重点 类固醇激素影响的细胞信号和调节网络 油菜素类固醇（BR），一种主要的生长促进激素，影响广泛的 发育和生理过程。利用遗传学，基因组， 生物化学和蛋白质组学方法在拟南芥模型系统中，我们有 阐明了BR与受体激酶BRI 1结合导致 通过进化保守的BSU 1家族激活转录因子BZR 1 磷酸酶和GSK 3样激酶。此外，我们还发现， 水平BR信号通路如何与许多其他信号通路整合 控制基因转录程序驱动细胞伸长的调控网络 以及各种具体的发展计划。我们正在进行的研究发现 BR信号通过许多翻译后机制直接调节细胞内 重组和代谢程序通过直接蛋白质-蛋白质相互作用， 翻译后修饰我们建议重点关注这些机制， 信号通路与主要的细胞活动，如膜运输，细胞 分裂和碳代谢。此外，我们还将研究BR信号是如何通过 与其他信号通路进行对话，以平衡新陈代谢与生长需求， 在植物发育中进行细胞分化。这项研究概述了 建议将继续使用遗传和蛋白质组学方法的组合，并将 显着推进我们对细胞信号分子机制的理解 集成和信息处理。

R01GM066258: Genetic Biochemical Studies of Plant Steroid Signaling. PI: Zhiyong Wang Modified Project Summary/Abstract Section The long-term goal of this project is to understand the molecular networks through which hormones, environmental factors, and nutrients together control plant growth and development. Plants have evolved robust cellular signaling systems to regulate growth and metabolism according to internal status and environmental conditions. Dissecting these plant regulatory systems is important not only for food security and environmental sustainability, but also for understanding cellular regulation in general, as many mechanisms are highly conserved in animals and plants. This research program focuses on the cellular signaling and regulatory network impinged upon by the steroid hormone brassinosteroid (BR), a major growth-promoting hormone that impact a wide range of developmental and physiological processes. Using a combination of genetic, genomic, biochemical, and proteomic approaches in the Arabidopsis model system, we have elucidated molecular mechanisms by which BR binding to receptor kinase BRI1 leads to activation of transcription factor BZR1 through the evolutionarily conserved BSU1 family of phosphatases and GSK3-like kinases. Furthermore, we have revealed at molecular level how the BR signaling pathway is integrated with many other signaling pathways into regulatory networks that control gene transcription program driving cell elongation and various specific developmental programs. Our ongoing research has uncovered many posttranslational mechanisms by which BR signaling directly modulates cellular reorganization and metabolic programs through direct protein-protein interactions and posttranslational modifications. We proposed to focus on these mechanisms that link signaling pathways with major cellular activities such as membrane trafficking, cell division, and carbon metabolism. In addition, we will investigate how BR signaling cross talks with other signaling pathways to balance metabolism with growth demand and to program cellular differentiation in plant development. The research outlined in this proposal will continue to use combinations of genetic and proteomic approaches and will significantly advance our understanding of the molecular mechanisms of cellular signal integration and information processing.