VISIBLE MOUSE EMBRYO DEVELOPMENT AND GENE EXPRESSION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 PSC和杜克体内显微镜中心正在合作 应用PSC的体积解剖可视化和分析工具 微型MRI小鼠数据集。 Micro-MRI数据将使用 杜克的MRI扫描仪在约30微米处捕获全部小鼠体积 在一系列的发展， 阶段和各种基因敲除。 数据将被重新组合 4D数据集，用于PSC的Volume Browser/Volume Server 用于配准、分割和解剖比较。 这项工作 正处于准备提案的原则证明阶段。 数据存储、可视化和分割处理需要高性能计算资源。 每个体积时间点 是1G字节的数据，我们预计每个标本有8到16个时间点。 最初，我们将与一个标本，但如果该项目 最终将扩大到数百个。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. PSC and the Duke Center for In Vivo Microscopy are collaborating to apply PSC's volumetric anatomy visualization and analysis tools to micro-MRI mouse data sets. Micro-MRI data will be captured using the MRI scanners at Duke to capture full mouse volumes at ~30micron resolution over a series of mouse speciments at a series of development stages and with various gene knockouts. Data will be reassembled into 4D datasets to be used with PSC's Volume Browser/Volume Server for registration, segmention and anatomical comparisons. This work is in the proof of principle stage for proposal preparation. High performance computing resources are needed for data storage, visualization and segmentation processing. Each volumetric time point is 1Gbyte of data and we anticipate 8 to 16 time points per specimen. Initially we will be working with one specimen but if the project is funded this will eventually expand to several hundred.