Resource Development Core

项目概要/摘要（资源开发核心） 资源开发核心将专注于开发技术，以帮助肾脏研究人员 实现定量，大规模显微镜作为了解肾功能的工具的潜力， 疾病组织清除的方法为研究人员提供了对整个小鼠肾脏进行成像的能力， 这提供了在细胞的完整表达的背景下识别和表征整个细胞群的机会。 三维组织和关系。然而，技术挑战阻碍了广泛的... 大规模、高分辨率显微镜作为一种有效的研究工具得到广泛应用。资源 开发核心将通过以下方式应对这些挑战：（1）开发独特的多尺度/多 分辨率的方法，大规模成像的啮齿动物和人类肾脏的基础上，一种新的混合开放， 顶部光片显微镜系统（2）自动图像分析的尖端方法的发展 以及（3）开发用于整合成像和转录组数据以进行组合分析的方法。 这里开发的方法将不断纳入分子成像核心的服务， 通过出版物、在线知识库和IU奥布莱恩显微镜讲习班传播。

Project Summary/Abstract (Resource Development Core) The Resource Development Core will focus on developing technologies to help renal investigators realize the potential of quantitative, large-scale microscopy as a tool for understanding kidney function and disease. Methods of tissue clearing provide researchers with the ability to image the entire mouse kidney, presenting the opportunity to identify and characterize the entire population of cells in the context of their full three-dimensional organization and relationship. However, there are technical challenges that hinder the wide- spread implementation of large-scale, high-resolution microscopy as an effective research tool. The Resource Development Core will address these challenges through (1) the development of a unique multi-scale/multi- resolution approach to large-scale imaging of the rodent and human kidney based upon a novel hybrid open- top lightsheet microscope system (2) the development of cutting-edge methods of automated image analysis and (3) the development of methods for integrating imaging and transcriptomic data for combined analyses. Methods developed here will be continuously incorporated into the services of the Molecular Imaging Core and disseminated in publications, online repositories and through the IU O’Brien Microscopy Workshop.