UCD Mutant Mouse Regional Resource Center

这是重新提交的第一个竞争性持续申请，请求NIH/NCRR支持 加州大学戴维斯分校突变小鼠区域资源中心(UCD-MMRRC)的未来5年。整体而言 UCD-MMRRC的目标是作为MMRRC国家计划的一个组成部分，接受， 维护和分发用于生物医学研究的突变小鼠品系。MMRRC计划 接受转基因、基因敲除和所有其他类型的突变小鼠品系，对捐赠者免费，并在 SPF-再衍生和超低温保存，从遗传上分布了种质资源，并在许多 以一小笔费用向要求调查人员提供非商业性、学术性的表型验证小鼠 仅供研究之用。UCD-MMRRC目前对9000个突变株进行了普查，这些突变株保持着 积极培育小鼠克隆、冷冻胚胎或种质，和/或胚胎干细胞克隆。 此外，UCD-MMRRC与其他3个区域MMRRC作为中心提供协调网络服务 卓越从事生物学研究和教学的实验鼠，提供科学的 拥有突变小鼠生物学、群体管理、常规和 先进的基因分型和表型鉴定能力、临床和解剖病理服务、菌株拯救、 传染病诊断和医疗保健等领域。为了与这一使命保持一致，该应用程序 国家4个相互关联的具体目标，重点是继续维持突变小鼠品系 资源，接受进入资源的新线路，研究和开发项目，最终将 被转移到资源，以提高其功能效率和能力，并最终提供 在老鼠生物学方面提供推广和教育培训的机会。这些具体的目标是促成的 由UCD比较医学中心(CCM)密切联系的教师专业知识，UCD小鼠 生物学项目(UCD-MBP)以及医学院和兽医学院。这些关系 允许利用MMRRC支持，从而扩大MMRRC的机会并使其受益 国家计划以及生物医学和行为科学研究界。

This is a resubmission of the first competitive continuing application requesting NIH/NCRR support for the next 5 years of the UC Davis Mutant Mouse Regional Resource Center (UCD-MMRRC). The overall objective of the UCD-MMRRC is to serve as a component of the MMRRC National Program, to accept, maintain, and distribute mutant mouse strains for use in biomedical research. The MMRRC program accepts transgenics, knockouts, and all other kinds of mutant mouse lines at no cost to the donor, and after SPF-rederivation and cryopreservation, distributes breeding stock orgermplasm of genetically, and in many cases phenotypically verified mice for a small fee to requesting investigators for non-commercial, academic research purposes only. The UCD-MMRRC currently has a census of >9000 mutant strains maintained as either actively breeding mousecolonies, frozen embryos or germplasm, and/or embryonic stem cell clones. Further, the UCD-MMRRC serves in a coordinated network with 3 other regional MMRRCs as a center of excellence engaged in research and teaching on the biology of the laboratory mouse, providing the scientific community with professional expertise in mutant mouse biology, colony management, conventional and advanced genotyping and phenotyping capabilities, clinical and anatomic pathology services, strain rescue, infectious disease diagnostics and health care, and other areas. In keeping with this mission, this application states 4 interconnected Specific Aims that focus on continuing maintenance of mutant mouse lines in the resource, acceptance of new lines into the resource, research and development projects that eventually will be transferred to the resource to improve its functional efficiency and capabilities, and finally to provide opportunities for outreach and education and training in mouse biology. These Specific Aims are facilitated by closely linked faculty expertise of the UCD Center for Comparative Medicine (CCM), the UCD Mouse Biology Program (UCD-MBP), and the Schools of Medicine and Veterinary Medicine. These relationships allow leveraging of MMRRCsupport, resulting in expanded opportunities for, and benefit to, the MMRRC National Program and the biomedical and behavioral scientific research community.