PROTEIN QUANTITATION USING MASS SPECTROMETRY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 质谱学是定量测定低丰度蛋白质和多肽的一种选择方法，已被应用于生物学的许多领域。我们正在开发一系列蛋白质和多肽定量的计算方法，包括寻找和整合感兴趣的峰的方法，以及检测干扰的方法，以获得对样品中存在的物质数量的稳健测量。 张宝明，B.M.Ueberheide，S.Waldemarson，S.Myung，K.Molloy，J.Eriksson，B.T.Chait，T.A.Neubert，D.Feny？，《蛋白质定量使用质谱学》，分子生物学方法，计算生物学，编辑。D.Feny？，Humana Press，新泽西州托托瓦，2010年出版。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mass spectrometry is a method of choice for quantifying low abundance proteins and peptides and it has been applied to many areas of biology. We are developing a range of computational methods for protein and peptide quantification, including methods for finding and integrating peaks of interest, and detecting interference to obtain a robust measure of the amount of material present in the samples. G. Zhang, B.M. Ueberheide, S. Waldemarson, S. Myung, K. Molloy, J. Eriksson, B.T. Chait, T.A. Neubert, and D. Feny¿, "Protein Quantitation Using Mass Spectrometry", Methods in Molecular Biology, Computational Biology, Ed. D. Feny¿, Humana Press, Totowa, NJ, in press 2010.