Spectrophotometric analysis system for determination of enzyme kinetics and metabolite concentrations

申请人的研究计划使用果蝇和鱼类来解决涉及复杂网络的分子遗传学，能量代谢和生态生理学的问题。 我们的研究计划是不同的，但桥梁部门和学科边界统一的酶功能和代谢的复杂性的中心重点。 虽然我们提出了不同的问题，但我们对酶动力学和代谢物浓度的准确定量有着共同的绝对要求，并且基本上需要高效快速地处理大量温度敏感的样品。 我们建议购买一套必要的设备，用于对大量样品进行酶活性和代谢物浓度测定。 这个包是围绕一个微量天平和一个微量板分光光度计，这两件设备是需要作为一个包为我们的研究。 这套设备将是唯一的劳伦特大学校园允许研究项目，否则不可能。 此外，该包将允许申请人的两个实验室的研究计划的延续和扩展，并允许在申请人和其他研究人员的监督下，在化学和生物化学和生物学部门的本科生和研究生的培训。

The applicants' research programs use fruit flies and fish to address questions involving the molecular genetics of complex networks, energy metabolism and ecological physiology.  Our research programs are distinct, but bridge departmental and discipline boundaries united by a central focus on enzyme function and metabolic complexity.  While we ask different questions, we share a common absolute requirement for the accurate quantification of enzyme kinetics and metabolite concentration, and fundamental need to efficiently and quickly process a large number of temperature-sensitive samples.  We propose to purchase a package of equipment necessary for spectrophotmetric assays of a large number of samples for a suite of enzyme activities and metabolite concentrations.  This package is built around a microbalance and a microplate spectrophotometer, two pieces of equipment that are required as a package for our research.  This package of equipment will be unique on the Laurentian University campus allowing research projects not otherwise possible.  Further, the package will allow continuation and expansion of the research programs of both laboratories of the applicants, and allow on the training of undergraduate and graduate students under the supervision of the applicants and other researchers in both Chemistry and Biochemistry and Biology Departments.