Sterilization of culture media/substrates by an autoclave at bamfield marine sciences centre

班菲尔德海洋科学中心(BMSC)需要一台独立的高压灭菌器，以供广泛的主要研究人员、博士后研究员、研究生、访问研究人员、本科生和研究助理使用。BMSC由艾伯塔大学、卡尔加里大学、不列颠哥伦比亚省大学、维多利亚大学和西蒙·弗雷泽大学拥有和运营。该高压灭菌器对于NSERC发现的基本研究项目至关重要，这些研究项目侧重于进化生物学、入侵物种、鱼类生理学、神经科学、发育和生殖生物学、分子生物学、海洋生态和保护生物学。此外，为保健食品市场开发海藻的应用研究项目也需要高压灭菌器。高压灭菌器将能够对培养物和海水介质、玻璃器皿、仪器以及可高压灭菌的塑料分配器和容器进行灭菌。高压灭菌器还将对BMSC大学项目部定期举办的分子生物学课程中使用的材料进行灭菌。高压灭菌器的重要特点是使用蒸馏水作为蒸汽源的独立能力。有足够的容量容纳几个两升容量的烧瓶，并且易于操作和维护。所要求的高压灭菌器取代了一台机器，该机器自1975年获得以来对大量调查人员进行的研究计划一直很重要，但现在无法使用。

A stand-alone autoclave sterilizer is requested for the Bamfield Marine Sciences Centre (BMSC), for multifaceted use by a broad range of principal investigators, post-doctoral fellows, graduate students, visiting researchers, undergraduates and research assistants.  BMSC is owned and operated by the Universities of Alberta, Calgary, British Columbia and Victoria and Simon Fraser University.  The autoclave is essential for basic NSERC Discovery research programs focused on evolutionary biology, invasive species, fish physiology, neuroscience, developmental and reproductive biology, molecular biology, marine ecology and conservation biology.  The autoclave is also needed for applied research programs on the development of marine algae for the health food market.  The autoclave will enable the sterilization of culture and seawater media, glassware, instruments and autoclavable plastic dispensers and containers.  The autoclave will also sterilize materials used on molecular biology courses periodically conducted by the University Programs department of BMSC.  Features of importance for the autoclave, is a stand-alone capability using distilled water as a steam source, adequate capacity to contain several two-litre volumetric flasks and ease of operation and maintenance.  The requested autoclave replaces a machine that has been important to the research programs conducted by large numbers of investigators since its acquisition in 1975, but is now non-serviceable.