Cell culture facilities

NSERC资助的2个研究项目和1个NSERC-CIHR合作健康研究项目所需的细胞培养设施，涉及生物化学、细胞信号、氧化应激、植物与分子生物学、细胞低温保存和环境毒理学，旨在了解：(1)哺乳动物(HeLa、A549、CHO、HepG2)和植物细胞对不同胁迫(氧化应激、热休克、农药)的细胞和分子响应；(2)植物耐低温的细胞、分子和遗传机制；小麦蛋白对肝细胞、分泌胰岛素的INS832/13细胞和胰岛的保护作用机制。这些项目都是在哺乳动物细胞中进行的，需要全天候和日常使用细胞培养设施。三洋科科萨菲二氧化碳孵化器采用2个堆叠室，具有二氧化碳精确度高、温度可控、污染可控等特点。单光束、双探测器红外(IR2)二氧化碳传感器提供精确的二氧化碳控制和开门后的快速恢复。直接热风采暖系统在微处理器的PID控制下精确调节温度。该保温箱具有三个特点，可降低细胞培养中的污染风险：i)SterisonicTM双氧水灭菌系统，用于现场全室内灭菌；ii)集成SafeCellTM UV系统，清洁内部气流，以消除空气中的污染物和潮湿的PAN污染物；iii)Incu SaFeTM富铜不锈钢内表面，确保杀菌保护。Thermolyne Locator JR Plus低温容器的存储容量为20箱，可容纳2000瓶细胞。有索引的机架和盒子系统允许快速高效地提取样品。有一个液氮水平的超声波监测器，以保护生物样本。为了控制传代次数和可能的细胞污染，必须在-196℃保存冰冻细胞系。在这两个由NSERC资助的加拿大和国际高级研究人员的实验室里，细胞培养设施对于高质量、竞争性的研究和研究生培训是不可或缺的。

The Cell culture facilities are requested for 2 NSERC-funded research programs and 1 NSERC-CIHR Collaborative Health Research Project in biochemistry, cell signaling, oxidative stress, plant and molecular biology, cell cryopreservation and environmental toxicology, which aim to understand: (1) the cellular and molecular responses to different stresses (oxidative stress, heat shock, pesticides) in mammalian (HeLa, A549, CHO, HepG2) and plant cells; (2) the cellular, molecular, and genetic mechanisms that regulate low temperature tolerance in plants; and (3) mechanisms involved in the protective function of wheat proteins against cryopreservation damage in hepatocytes, insulin-secreting INS832/13 cells and pancreatic islets. These projects are all carried out in mammalian cells and require access to cell culture facilities on a fulltime and daily basis. The Sanyo Incusafe CO2 incubator with 2 stackable chambers has features of precision of CO2 and temperature control, and contamination control. The single-beam, dual detector infrared (IR2) CO2 sensor delivers precise CO2 control and quick recovery following door openings. The Direct Heat & Air JacketTM Heating System precisely regulates temperature under microprocessor PID control. The incubator has three features to decrease risk of contamination in cell cultures: i) A SterisonicTM H2O2 sterilization system for total chamber sterilization in situ; ii) An integrated SafeCellTM UV system cleans interior airflow to destroy airborne and humidity pan contaminants; iii) InCu SaFeTM copper-enriched stainless steel interior surfaces assure germicidal protection. The Thermolyne Locator Jr Plus cryogenic container has a storage capacity of 20 boxes and holds 2000 vials of cells. An indexed rack and box system allows quick and efficient sample retrieval. There is an ultrasound monitor for liquid nitrogen levels, to safeguard biological samples. It is essential to stock frozen cell lines at -196C to control passage number and possible cell contaminations. The cell culture facilities are indispensable for high quality, competitive research and graduate student training in the laboratories of these 2 senior NSERC-funded investigators, at the Canadian and international levels.