UV-visible spectrophotometer for specific biochemical utility

紫外可见吸收光谱是生物化学和生物物理学中一种功能强大且用途广泛的技术，而紫外可见光谱仪是任何分子生物学/生物化学系中不可缺少的设备，不仅需要对核酸和蛋白质进行定量和表征，而且还需要测量酶动力学、结构转变以及配体与生物大分子的相互作用。西蒙·弗雷泽大学分子生物学和生物化学系的学生和教职员工可以使用专用的荧光和圆二色(CD)分光光度计，但不能使用功能齐全的UV-VIS分光光度计。因此，这个由大约30名教员和100名研究生组成的强烈研究导向的系，无法可靠地接触到像高质量UV-Vis分光光度计这样的基本研究设备。最关键的是，由于缺乏能够满足这些研究人员在生物化学和分子生物学方面特别定制的需求的UV-VIS分光光度计，该申请的PI和共同申请者的研究计划正受到阻碍，例如使用放射性标记的样品、混浊的样品和含有活体的样品，例如

UV-Visible Absorption Spectroscopy is a powerful and versatile technique in biochemistry and biophysics, and a UV-Vis Spectrophotometer is an indispensable piece of equipment in any Molecular Biology / Biochemistry department, required not only for quantitation and characterization of nucleic acids and proteins, but also for the measurement of enzyme kinetics, structural transitions, and the interaction of ligands with bio-macromolecules. The students and faculty in the Simon Fraser University Department of Molecular Biology and Biochemistry have access to dedicated Fluorescence and Circular Dichroism (CD) spectrophotometers but not to a functional UV-Vis spectrophotometer. As a consequence, this strongly research-oriented department, comprising of ~30 faculty and > 100 graduate students, has no reliable access to such a fundamental piece of research equipment as a high quality UV-Vis spectrophotometer. Most critically, the research programs of the PI and co-applicants of this application are being hampered from lack access to a UV-Vis spectrophotometer that is able to meet the specifically tailored needs of these researchers in biochemistry and molecular biology, such as the use of radio-labeled samples, turbid samples, and samples containing live organisms, such as