Optimize Fermentation Process to Produce PHA for the Application of Biodegradable Plastics

石油基塑料对环境的影响，特别是海洋和水生环境中的塑料污染，已经引起了国际社会的关注，并导致许多司法管辖区制定政策来解决这一重大环境灾难。因此，加拿大政府已承诺到2025年实现零塑料废物，包括最早在2021年禁止使用一次性塑料，减少塑料污染，并让公司对其废物负责。 Metagenom生物生命科学公司是一家位于安大略省滑铁卢的创新研究公司。Metagenom Bio为客户的微生物组分析需求提供方便快捷的端到端解决方案。其产品和服务包括DNA提取试剂盒、诊断测序、生物信息学分析和微生物组改进产品。它们的应用涵盖农业、食品安全、生命科学和学术研究。 Metagenom Bio有兴趣生产混合的短链长度/中链长度聚羟基链烷酸酯（PHA）共聚物，该共聚物可以使用其基因组工程改造的恶臭假单胞菌菌株替代食品包装和饮料瓶中使用的传统塑料。通过与兰顿学院的合作项目，Metagenom Bio旨在展示和改进通过细菌细胞发酵生产聚羟基链烷酸酯生物塑料，使用挥发性脂肪酸（VFA）代表沥滤床反应器沥滤液作为碳源。 可生物降解的生物塑料是加拿大政府减少塑料污染以实现零塑料废物目标的解决方案。该项目的研究成果将为从食物垃圾中生产生物塑料的进一步商业化奠定基础，并提供数据支持Metagenom Bio开发商业案例，以进一步扩大这一概念。

The environmental impact of petroleum-based plastics, highlighted by plastic pollution in marine and aquatic environments, has garnered international attention, and has resulted in many jurisdictions developing policies to address this major environmental disaster. As such, the Canadian government has pledged to achieve zero plastic waste by 2025, including a ban of single-use plastics as early as 2021, reducing plastic pollution, and making companies responsible for their waste. Metagenom Bio Life Science Inc. is an innovative research company located in Waterloo, ON. Metagenom Bio provides convenient and rapid end-to-end solutions for clients' microbiome analysis needs. Their products and services include DNA extraction kits, diagnostic sequencing, bioinformatics analysis and microbiome improvement products. Their applications cover agriculture, food safety, life science and academic research. Metagenom Bio is interested in producing mixed short chain length / medium chain length polyhydroxyalkanoate (PHA) copolymer that can substitute for traditional plastic used in food packaging and beverage bottles using their genome engineered Pseudomonas putida strains. Through a collaborative project with Lambton College, Metagenom Bio aims to to demonstrate and refine the production of polyhydroxyalkanoate bioplastic by bacterial cell fermentation, using volatile fatty acids (VFA) representing leach bed reactor leachate, as carbon source. Biodegradable bioplastics are a solution to the Government of Canada's stated goal of reducing plastic pollution to achieve zero plastic waste. The research results of this project will provide a foundation for the further commercialization of bioplastics production from food waste, with data to support the development of a business case by Metagenom Bio for further scale up of the concept.