A time, spatial and phase domain gated multispectral imaging for diagnostics and therapy monitoring

用于诊断和治疗监测的时间、空间和相域门控多光谱成像 申请者的研究项目是生物光子学领域，该领域利用光进行诊断和治疗。光学诊断的优势是最小或非侵入性的相互作用，同时提供关于生物组织的结构、代谢和分子变化的重要信息。光疗和手术的使用最大限度地减少了对非靶向组织的破坏，并且不涉及与电离辐射的相互作用。总之，生物光子学为患者护理提供了先进的、在某些情况下还是独特的解决方案。申请者的研究项目集中在成像诊断、治疗反馈和监测方面。 拟议的研究策略集中在表面和内窥镜应用，利用多光谱时间、空间和相位选通成像技术，其中图像的每个像素通过对吸收、散射、折射率、荧光和拉曼特性的解释来提供关于组织形态和新陈代谢的信息。提出的研究有条件地可分为三个方向：(1)结合荧光和拉曼显微内窥镜进行间质光学活检(目前主要应用于乳房外科)；(2)空间门控成像用于高散射介质的深度询问(目前主要应用于皮肤微循环形态)；(3)大视场多光谱时域全息干涉成像(目前主要应用于抗生素-细菌反应和体外光动力学反应的即时监测)。 在我第一次申请Discovery后的过去5年里： 我的团队开发和发明了许多新的研究模式，3名博士生和2名硕士生已经完成，1我的博士后成为Ryerson Biomedzone Oxilight公司的首席执行官和总裁，目前的投资和资金超过20万，我的博士生中有1人接受了苏黎世大学助理教授的职位，目前我的团队包括3名博士生，5名硕士生和4名非退化研究助理。我与我的学生和波斯托克一起出版了27本同行评议的出版物，6本书的章节，20个诉讼程序，提交和提交了4项专利申请。我获得了5次客座教授的任命(德国慕尼黑工业大学、德国厄兰根-纽伦堡大学、法国蒙彼利埃大学、日本鹿儿岛大学、葡萄牙阿维罗大学)，客座编辑1期期刊(照片医学)，接受1次邀请进入编辑委员会(生物医学光子学)，在加拿大、美国、德国、法国、日本、以色列、乌克兰、俄罗斯、葡萄牙主持了15次主旨演讲和特邀讲座，主持了4次会议，并参加了5个会议计划委员会。

A time, spatial and phase domain gated multispectral imaging for diagnostics and therapy monitoring The applicant's research program is in the area of biophotonics, where light is employed for diagnostics and therapy. The advantage of optical diagnostics is minimally or noninvasive interaction while providing important information about structural, metabolic and molecular changes in biotissues. Usage of light therapy and surgery minimizes destruction of non-targeted tissues and does not involve interaction with ionizing radiation. Altogether, biophotonics proposes advanced and in some cases unique solutions for patient care. The applicant's research program is focused on imaging diagnostics, treatment feedback and monitoring. The proposed research strategy concentrates on superficial and endoscopic applications, exploiting multispectral time, spatial and phase gated imaging technologies where each pixel of the image provides information about tissue morphology and metabolism via interpretation of absorption, scattering, refraction index, fluorescence and Raman properties. The proposed research can be conditionally divided into 3 directions: (1) combined fluorescence and Raman microendoscopy for interstitial optical biopsy (currently the main application is the breast surgery); (2) spatial gated imaging for deep interrogation of highly scattering media (currently the main application is skin micro-circulation morphology); (3) a large field of view multi-spectral time domain holographic interferometry imaging (currently the main applications are instant monitoring of antibiotic-bacterial reactions and photodynamic reactions in vitro). During the last 5 years since I applied to my first Discovery: A number of new research modalities were developed and invented in my group, 3 my PhD students and 2 Master students have already finished, 1 my postdoc has become CEO and President of the company Oxilight at Ryerson Biomedzone with current level of investments and funding more than 200K, 1 of my PhD students accepted the position of Assistant Professor in Zurich University, and currently my group includes 3 PhDs, 5 Master students and 4 undegrad research assistants. I have published with my students and postoc 27 peer-reviewed publications, 6 book chapters, 20 proceedings, filed and submitted 4 patent applications. I obtained 5 appointments as a visiting professor (Technical University of Munich, Germany, University Erlangen-Nuremberg, Germany; University of Montpellier, France; Kagoshima University, Japan; University of Aveiro, Portugal), guest edited 1 journal issue (Photomedicine), accepted 1 invitation into Editorial Board (Biomedical Photonics) , conducted 15 key-note and invited lectures in Canada, USA, Germany, France, Japan, Israel, Ukraine, Russia, Portugal, chairing 4 conferences and participated in 5 conference program committees.